Overview

  • Product nameADP/ATP Ratio Assay Kit (Bioluminescent)
  • Tests
    200 x 1 test
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay typeSemi-quantitative
  • Sensitivity
    < 100 cells/well
  • Assay time
    0h 30m
  • Product overview

    Abcam's ADP/ATP Ratio Assay Kit (Bioluminescent) utilizes bioluminescent detection of the ADP and ATP levels for a rapid screening of apoptosis, necrosis, growth arrest, and cell proliferation simultaneously in mammalian cells. The assay utilizes the enzyme luciferase to catalyze the formation of light from ATP and luciferin, and the light can be measured using a luminometer or Beta Counter. ADP level is measured by its conversion to ATP that is subsequently detected using the same reaction. The assay can be fully automatic for high throughput and is highly sensitive (detects 100 mammalian cells/well).
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    The changes in ADP/ATP ratio have been used to differentiate the different modes of cell death and viability. Increased levels of ATP and decreased levels of ADP have been recognized in proliferating cells. In contrast, decreased levels of ATP and increased levels of ADP are recognized in apoptotic cells. The decrease in ATP and increase in ADP are much more pronounced in necrosis than apoptosis.

  • Tested applicationsFunctional Studiesmore details

Properties

  • RelevanceThe changes in ADP/ATP ratio have been used to differentiate the different modes of cell death and viability. Increased levels of ATP and decreased levels of ADP have been recognized in proliferating cells. In contrast, decreased levels of ATP and increased levels of ADP are recognized in apoptotic cells. The decrease in ATP and increase in ADP are much more pronounced in necrosis than apoptosis.

Applications

Our Abpromise guarantee covers the use of ab65313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Protocols

References for ADP/ATP Ratio Assay Kit (Bioluminescent) (ab65313)

This product has been referenced in:
  • Ramadasan-Nair R  et al. Mitochondrial alterations and oxidative stress in an acute transient mouse model of muscle degeneration: implications for muscular dystrophy and related muscle pathologies. J Biol Chem 289:485-509 (2014). Read more (PubMed: 24220031) »
  • Jamwal S  et al. Characterizing virulence-specific perturbations in the mitochondrial function of macrophages infected with Mycobacterium tuberculosis. Sci Rep 3:1328 (2013). Functional Studies . Read more (PubMed: 23435464) »

See all 12 Publications for this product

Product Wall

Very sensitive and easy to use

Excellent Excellent 5/5 (Ease of Use)
Abreviews
We have used this product extensively to measure changes in the ATP/ADP ratio in response to changes in glucose availability and during stress tests (H2O2 treatment). We have some recommendations below for use:

1) Use a black-walled 96-well plate to reduce plate phosphorescence
2) If possible, use ~1,000 cells per well. If you can do a seeding density curve, you will notice that the ATP/ADP ratio drops with increasing cell number. This is due to glucose depletion for the media. Less cells will give you a better charged cell.
3) Following on from point 2, change the media 1-2 hours before starting your experiment. This will replenish the glucose in the media. We found this to be very important when trying to utilise physiological glucose concentrations (2.5-10 mM glucose).

Reliable kit and easy to use.
Username

Dr. Craig Beall

Verified customer

Submitted Sep 17 2014

The signal should be fairly stable as long as the sample is protected from light. But we suggest measuring after 2 mins to make sure that no light is quenched. Waiting for 10 mins is not advisable since some light can be quenched during this time. For ...

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We can recommend two protocols:
1. Prepare a single cell suspension from the tissues of interest with any method desired (cells have to stay intact). Then treat the cells as instructed in the protocols section for adherent cells: Remove cultur...

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Please see the following publication which may be of use to you:

Diguet N et al. Muscle creatine kinase deficiency triggers both actin depolymerization and desmin disorganization by advanced glycation end products in dilated cardiomy...

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Thank you for your inquiry.

I am happy to confirm that ab65313 can be used with mouse liver tissue samples.

This kit can be used to measure the ADP/ATP Ratio, AMP is not measured.

If you are planning to use this kit with t...

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Yes, you are correct. Lower ATP readings would indicate a decrease in cell viability.

Ab65314 is recommended for cell viability. For media, use the same protocol as mentioned for the suspension cells in step 2.a.



1. If you are using adherent cells, than yes, you will get a total volume of 150ul.

2. Will have to ask the lab for this, as it not clear from the protocol if more or less buffer is needed. I will get back to you as soon as I recei...

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Vielen Dank für Ihren Anruf.

Ich habe nun Nachricht vom Labor erhalten mit mehr Informationen über die Verarbeitung von Gewebe für dieses Kit:

Es muss eine Single-Cell-Suspension aus Gewebe hergestellt werden.
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Thank you for contacting us.

No, there are no ATPase Inhibitors in the buffers. The ATP is very labile and hence the reaction must be carried out within the set timeframe. Yes, you can make standard curves with pure ATP/ADP.


I...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"