ADP/ATP Ratio Assay Kit (Bioluminescent) (ab65313)
- Product nameADP/ATP Ratio Assay Kit (Bioluminescent)
- Tests100 x 1 test
- Sample typeUrine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
- Assay typeSemi-quantitative
- Sensitivity< 100 cells/well
- Assay time0h 30m
- Product overview
Abcam's ADP/ATP Ratio Assay Kit (Bioluminescent) utilizes bioluminescent detection of the ADP and ATP levels for a rapid screening of apoptosis, necrosis, growth arrest, and cell proliferation simultaneously in mammalian cells. The assay utilizes the enzyme luciferase to catalyze the formation of light from ATP and luciferin, and the light can be measured using a luminometer or Beta Counter. ADP level is measured by its conversion to ATP that is subsequently detected using the same reaction. The assay can be fully automatic for high throughput and is highly sensitive (detects 100 mammalian cells/well).
Visit our FAQs page for tips and troubleshooting.
The changes in ADP/ATP ratio have been used to differentiate the different modes of cell death and viability. Increased levels of ATP and decreased levels of ADP have been recognized in proliferating cells. In contrast, decreased levels of ATP and increased levels of ADP are recognized in apoptotic cells. The decrease in ATP and increase in ADP are much more pronounced in necrosis than apoptosis.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 200 tests ADP Converting Enzyme (Lyophilised) Blue Cap 1 vial ATP Monitoring Enzyme (Lyophilised) 1 vial Enzyme Reconstitution Buffer 1 x 2.15ml Nucleotide Releasing Buffer 1 x 50ml
- Research Areas
Our Abpromise guarantee covers the use of ab65313 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for ADP/ATP Ratio Assay Kit (Bioluminescent) (ab65313)
This product has been referenced in:
- Fu M et al. Hydrogen sulfide (H2S) metabolism in mitochondria and its regulatory role in energy production. Proc Natl Acad Sci U S A : (2012). Read more (PubMed: 22323590) »
- Li L & Zuo Z Isoflurane postconditioning induces neuroprotection via Akt activation and attenuation of increased mitochondrial membrane permeability. Neuroscience 199:44-50 (2011). Read more (PubMed: 22040798) »