Overview

  • Product nameADP/ATP Ratio Assay Kit (Bioluminescent)
  • Tests
    200 x 1 test
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay typeSemi-quantitative
  • Sensitivity
    < 100 cells/well
  • Assay time
    0h 30m
  • Product overview

    Abcam's ADP/ATP Ratio Assay Kit (Bioluminescent) utilizes bioluminescent detection of the ADP and ATP levels for a rapid screening of apoptosis, necrosis, growth arrest, and cell proliferation simultaneously in mammalian cells. The assay utilizes the enzyme luciferase to catalyze the formation of light from ATP and luciferin, and the light can be measured using a luminometer or Beta Counter. ADP level is measured by its conversion to ATP that is subsequently detected using the same reaction. The assay can be fully automatic for high throughput and is highly sensitive (detects 100 mammalian cells/well).
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    The changes in ADP/ATP ratio have been used to differentiate the different modes of cell death and viability. Increased levels of ATP and decreased levels of ADP have been recognized in proliferating cells. In contrast, decreased levels of ATP and increased levels of ADP are recognized in apoptotic cells. The decrease in ATP and increase in ADP are much more pronounced in necrosis than apoptosis.

  • Tested applicationsFunctional Studiesmore details

Properties

  • RelevanceThe changes in ADP/ATP ratio have been used to differentiate the different modes of cell death and viability. Increased levels of ATP and decreased levels of ADP have been recognized in proliferating cells. In contrast, decreased levels of ATP and increased levels of ADP are recognized in apoptotic cells. The decrease in ATP and increase in ADP are much more pronounced in necrosis than apoptosis.

Applications

Our Abpromise guarantee covers the use of ab65313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Protocols

References for ADP/ATP Ratio Assay Kit (Bioluminescent) (ab65313)

This product has been referenced in:
  • Ramadasan-Nair R  et al. Mitochondrial alterations and oxidative stress in an acute transient mouse model of muscle degeneration: implications for muscular dystrophy and related muscle pathologies. J Biol Chem 289:485-509 (2014). Read more (PubMed: 24220031) »
  • Ran C  et al. Proliferation-independent control of tumor glycolysis by PDGFR-mediated AKT activation. Cancer Res 73:1831-43 (2013). Read more (PubMed: 23322009) »

See all 11 Publications for this product

Product Wall



Please see the following publication which may be of use to you:

Diguet N et al. Muscle creatine kinase deficiency triggers both actin depolymerization and desmin disorganization by advanced glycation end products in dilated cardiomy...

Read More

Thank you for your inquiry.

I am happy to confirm that ab65313 can be used with mouse liver tissue samples.

This kit can be used to measure the ADP/ATP Ratio, AMP is not measured.

If you are planning to use this kit with t...

Read More


Yes, you are correct. Lower ATP readings would indicate a decrease in cell viability.

Ab65314 is recommended for cell viability. For media, use the same protocol as mentioned for the suspension cells in step 2.a.



1. If you are using adherent cells, than yes, you will get a total volume of 150ul.

2. Will have to ask the lab for this, as it not clear from the protocol if more or less buffer is needed. I will get back to you as soon as I recei...

Read More

Vielen Dank für Ihren Anruf.

Ich habe nun Nachricht vom Labor erhalten mit mehr Informationen über die Verarbeitung von Gewebe für dieses Kit:

Es muss eine Single-Cell-Suspension aus Gewebe hergestellt werden.
Read More

Thank you for contacting us.

No, there are no ATPase Inhibitors in the buffers. The ATP is very labile and hence the reaction must be carried out within the set timeframe. Yes, you can make standard curves with pure ATP/ADP.


I...

Read More



1) Yes, standard protein quantitation assays can be performed with extracts with the NRS buffer.

2) We have not tested for arsenic influence, but if you are using arsenic only to induce apoptosis, then it should not interfere with th...

Read More

Thank you for contacting us.

I can confirm that the ADP/ATP Ratio Assay Kit (Bioluminescent) - ab65313 is guaranteed to work with tissue extracts like heart tissue.

The Kit is likely to work with frozen tissue. However, we recommend t...

Read More

Thank you for contacting us.

This kit may be applicable to your studies, but I'd recommend looking in the literature for the best, most widely used way to distinguish between aerobic respiration and glycolysis. Abcam's ADP/ATP Ratio Assay Ki...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"