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ADP/ATP Ratio Assay Kit (Bioluminescent) (ab65313)

Q&A (34)Specific References (7)

Overview

  • Product nameADP/ATP Ratio Assay Kit (Bioluminescent)
  • Tests
    100 x 1 test
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay typeSemi-quantitative
  • Sensitivity
    < 100 cells/well
  • Assay time
    0h 30m
  • Product overview

    Abcam's ADP/ATP Ratio Assay Kit (Bioluminescent) utilizes bioluminescent detection of the ADP and ATP levels for a rapid screening of apoptosis, necrosis, growth arrest, and cell proliferation simultaneously in mammalian cells. The assay utilizes the enzyme luciferase to catalyze the formation of light from ATP and luciferin, and the light can be measured using a luminometer or Beta Counter. ADP level is measured by its conversion to ATP that is subsequently detected using the same reaction. The assay can be fully automatic for high throughput and is highly sensitive (detects 100 mammalian cells/well).
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    The changes in ADP/ATP ratio have been used to differentiate the different modes of cell death and viability. Increased levels of ATP and decreased levels of ADP have been recognized in proliferating cells. In contrast, decreased levels of ATP and increased levels of ADP are recognized in apoptotic cells. The decrease in ATP and increase in ADP are much more pronounced in necrosis than apoptosis.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceThe changes in ADP/ATP ratio have been used to differentiate the different modes of cell death and viability. Increased levels of ATP and decreased levels of ADP have been recognized in proliferating cells. In contrast, decreased levels of ATP and increased levels of ADP are recognized in apoptotic cells. The decrease in ATP and increase in ADP are much more pronounced in necrosis than apoptosis.

    • Applications

    Our Abpromise guarantee covers the use of ab65313 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Protocols

    References for ADP/ATP Ratio Assay Kit (Bioluminescent) (ab65313)

    This product has been referenced in:
    • Fu M  et al. Hydrogen sulfide (H2S) metabolism in mitochondria and its regulatory role in energy production. Proc Natl Acad Sci U S A : (2012). Read more (PubMed: 22323590) »
    • Li L & Zuo Z Isoflurane postconditioning induces neuroprotection via Akt activation and attenuation of increased mitochondrial membrane permeability. Neuroscience 199:44-50 (2011). Read more (PubMed: 22040798) »

    See all 7 Publications for this product

    Publishing research using ab65313 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab65313 Submit a Question for ab65313

    Displaying 1 - 10 of 34 results for Abreviews and Q&A

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    ADP/ATP Ratio Assay Kit (Bioluminescent) (ab65313)
    Hello. I would like to know if I can ue your kit to measure ATP
    content in mouse liver.
    I need to measure heaptic ATP, ADP, and AMP. Any suggestion?
    Thank you,

    Thank you for your inquiry.

    I am happy to confirm that ab65313 can be used with mouse liver tissue samples.

    This kit can be used to measure the ADP/ATP Ratio, AMP is not measured.

    If you are planning to use this kit with tiss...

    Read More

    I see that the readout would be ATP in the media; this means that non-viable (or dead) cells would have lower ATP readings in the media compared to live cells.


    Yes, you are correct. Lower ATP readings would indicate a decrease in cell viability.

    Can you please suggest a product for which I can test cell viability using cell supernatants?

    Ab65314 is recommended for cell viability. For media, use the same protocol as mentioned for the suspension cells in step 2.a.

    I would like to ask you some questions about ADP/ATP Ratio Assay Kit (Bioluminescent) ab65313.

    1- Do I need to add totally 150



    µ

    Normal
    0




    false
    false
    false

    Read More



    1. If you are using adherent cells, than yes, you will get a total volume of 150ul.

    2. Will have to ask the lab for this, as it not clear from the protocol if more or less buffer is needed. I will get back to you as soon as I receive ...

    Read More

    Protokoll für Gewebe

    Vielen Dank für Ihren Anruf.

    Ich habe nun Nachricht vom Labor erhalten mit mehr Informationen über die Verarbeitung von Gewebe für dieses Kit:

    Es muss eine Single-Cell-Suspension aus Gewebe hergestellt werden.

    ...

    Read More


    Inquiry: Hi! Does this kit AB 65313 contain ATPase inhibitors in the buffers? Can we expect the levels of ATP within samples to be stabilized by the buffers? or in other words, how long will the ATP levels be stable from time of cell sampling to time...

    Read More

    Thank you for contacting us.

    No, there are no ATPase Inhibitors in the buffers. The ATP is very labile and hence the reaction must be carried out within the set timeframe. Yes, you can make standard curves with pure ATP/ADP.


    I ho...

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    for ab65313:
    1) can BCA assay be used to measure the protein concentration after using the kit's releasing buffer?
    2) samples contain up to 2 uM arsenate - quenching of luciferase possible
    3) can a standard curve for ADP and ATP be build using...

    Read More



    1) Yes, standard protein quantitation assays can be performed with extracts with the NRS buffer.

    2) We have not tested for arsenic influence, but if you are using arsenic only to induce apoptosis, then it should not interfere with the r...

    Read More

    Inquiry: Hi, We are considering whether this product would be suitable for our samples. We use heart tissue that has been snap-frozen in liquid nitrogen. Do you know if this would be a problem for this assay? Thank you

    Thank you for contacting us.

    I can confirm that the ADP/ATP Ratio Assay Kit (Bioluminescent) - ab65313 is guaranteed to work with tissue extracts like heart tissue.

    The Kit is likely to work with frozen tissue. However, we recommend to u...

    Read More

    I am trying to measure metabolic phenotypes in lymphocytes - specifically whether aerobic respiration is occurring vs. simply glycolysis. Others have used levels of ATP as a confirmation of each pathway (high ATP indicates respiration, while lower ATP ind...

    Read More

    Thank you for contacting us.

    This kit may be applicable to your studies, but I'd recommend looking in the literature for the best, most widely used way to distinguish between aerobic respiration and glycolysis. Abcam's ADP/ATP Ratio Assay Kit (...

    Read More

    I am using a Abcam ADP/ADP ratio assay kitits catalogue number is ab65313, lot number GR12038-2 . I refer the manual of that kit but for the ATP and ADP measurements there is units and calculations are given. the value i get after the luminescencewhat it ...

    Read More



    Unfortunately, this kit only aims to give you an overview of the ADP/ATP ratio to make a statement about the cell fate (cell viability assay)of your samples.

    As this is a Semi-quantitative test, you can only measure if A and/or B is the...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"