Anti-AE binding protein 1 antibody (ab54820)

Overview

  • Product name
    Anti-AE binding protein 1 antibody
    See all AE binding protein 1 primary antibodies
  • Description
    Mouse monoclonal to AE binding protein 1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 912-1014 of Human AE binding protein 1

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

Applications

Our Abpromise guarantee covers the use of ab54820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notes
    IHC-P: Use at a concentration of 3 µg/ml.
    WB: Use at a concentration of 1 - 5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      AEBP1 (adipocyte enhancer binding protein 1) is a transcriptional repressor which binds to the adipocyte enhancer 1 (AE-1) regulatory sequence, located in the proximal promoter region of the adipose P2 (aP2) gene. AEBP1 has carboxypeptidase (CP) activity and the direct binding of DNA to AEBP1 enhances its protease activity. AEBP1 expression is largely restricted to the aorta, particularly to smooth muscle cells.
    • Database links
    • Alternative names
      • ACLP antibody
      • Adipocyte enhancer binding protein 1 antibody
      • AEBP 1 antibody
      • AEBP1 antibody
      • Aortic carboxypeptidase like protein ACLP antibody
      • Aortic carboxypeptidase like protein antibody
      • FLJ33612 antibody
      see all

    Images

    • Western blot against tagged recombinant protein immunogen using ab54820 AE binding protein 1 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
    • ab54820 at 3 ug/ml staining AE binding protein 1 in human adrenal gland tissue section by Immunohistochemistry (Formalin /PFA fixed paraffin embedded tissue sections).

    References

    ab54820 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Aortic smooth muscle)
    Loading amount
    20 µg
    Specification
    Aortic smooth muscle
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 18 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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