Anti-AE2 antibody (ab226829)
Key features and details
- Rabbit polyclonal to AE2
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AE2 antibody
See all AE2 primary antibodies -
Description
Rabbit polyclonal to AE2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan -
Immunogen
Synthetic peptide within Human AE2 aa 1-50. The exact sequence is proprietary. (NP_001186621.1).
Database link: P04920 -
Positive control
- WB: HeLa and HEK-293T whole cell lysates. IP: HEK-293T whole cell lysate. IHC-P: Human ovarian carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab226829 was affinity purified using an epitope specific to AE2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 137 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 137 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Plasma membrane anion exchange protein of wide distribution. -
Sequence similarities
Belongs to the anion exchanger (TC 2.A.31) family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6522 Human
- Entrez Gene: 100172030 Orangutan
- Omim: 109280 Human
- SwissProt: P04920 Human
- SwissProt: Q5RD44 Orangutan
- Unigene: 647069 Human
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Alternative names
- AE 2 antibody
- AE2 anion exchanger antibody
- Anion exchange protein 2 antibody
see all
Images
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Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for AE2 using ab226829 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.
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All lanes : Anti-AE2 antibody (ab226829) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 137 kDa
Exposure time: 30 secondsLysates were prepared using RIPA lysis buffer.
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AE2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using RIPA lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226829 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226829 at 1 µg/ml.
Lane 1: ab226829 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab226829 has not yet been referenced specifically in any publications.