![Western blot - AEBP2 antibody [2012C4a] (ab74517) image](#ab74517.jpg)
Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Overview
Product name
Anti-AEBP2 antibody [2012C4a]
See all AEBP2 products (2) ...
Description
Mouse monoclonal [2012C4a] to AEBP2
Tested applications
WB, Dot Blotmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment from the N-terminal of human AEBP2.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
ab74517 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and then filtered through a 0.22µm membrane.
Clonality
Monoclonal
Clone number
2012C4a
Isotype
IgG1
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> PRC2
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
-
Western blot - AEBP2 antibody [2012C4a] (ab74517)
![Western blot - AEBP2 antibody [2012C4a] (ab74517) image](/ps/datasheet/Images/74/ab74517/ab74517.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab74517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 54 kDa.
Dot: Use at an assay dependent dilution.
Target
Function
DNA-binding transcriptional repressor. May interact with and stimulate the activity of the PRC2 complex, which methylates 'Lys-9' and 'Lys-27' residues of histone H3.
Sequence similarities
Belongs to the AEBP2/jing C2H2-type zinc-finger family.
Contains 3 C2H2-type zinc fingers.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ6ZN18
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Adipocyte enhancer binding protein 2 antibody
- Adipocyte enhancer-binding protein 2 antibody
- AE (adipocyte enhancer) binding protein 2 antibody
see all
Database links
- Entrez Gene: 121536 Human
- SwissProt: Q6ZN18 Human
- Unigene: 126497 Human
Anti-AEBP2 antibody [2012C4a] images:
Western blot - AEBP2 antibody [2012C4a] (ab74517)
Anti-AEBP2 antibody [2012C4a] (ab74517) + Immunising recombinant protein
Predicted band size : 54 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
References for Anti-AEBP2 antibody [2012C4a] (ab74517)
ab74517 has not yet been referenced specifically in any publications.
Publishing research using ab74517? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"