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Overview

  • Product nameAEC Single/Plus (30ml)
  • General notesAEC Single/Plus is a single, highly stable AEC chromogen/substrate working solution. When used in conjugation with immunoperoxidase detection systems, AEC produces a red colored and product at positive sites, yeilding strong contrast when combined with a blue hematoxylin counterstain. Specimens stained using AEC Single/Plus cannot be dehydrated in ethanol and must be mounted in an aqueous-based mounting medium.

    AEC Single/Plus (ab103742) is a single, stable ready for use solution. It can be used up until the expiry date so reduces waste when compared to DAB substrate/chromgen solutions. The solution is light sensitive and must be protected from exposure to light and stored in an opaque bottle or dark environment.

    Protocol
    • Once section have been incubated with peroxidase, wash with wash buffer.
    • Wipe slides removing excess buffer. Add enough drops of AEC Single/Plus to cover tissue sections.
    • Incubate for 5-15 minutes at room temperature. For optimal results, observe reaction under the microscope for signal developmant. Once the desired signal to noise ratio is acheived, stop the reaction by washing the slides in DI H2O.

    • Properties

  • Storage instructionsStore at +4°C in the dark.
  • Storage bufferConstituent: 6% Hydrogen peroxide
  • Concentration information loading...
  • Research Areas

    • AEC Single/Plus (30ml) images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AEC Single/Plus (30ml) (ab103742)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AEC Single/Plus (30ml) (ab103742)
      AEC Single/Plus staining Cytokeratin 5 & 14 in tonsil tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    References for AEC Single/Plus (30ml) (ab103742)

    ab103742 has not yet been referenced specifically in any publications.

    Publishing research using ab103742 ? Please let us know so that we can cite the reference in this datasheet

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    Please, Could you send me compliance certificate

    AB6908 GR55986
    AB6900 GR54072
    AB6886 GR14049
    AB103742 GR66527
    AB7413 GR64347

    Thank you for contacting us.

    As requested, I have attached the Certificates of Compliance for the antibodies and reagents you mentioned - to this email.

    I hope this information is helpful to you. Please do not hesitate to contact us ...

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    Hello,I want to buy substrats for Alkaline phosphatase and HRP for double elispot test.I have seen that you have for alkaline phosphatase  BCIP /NBT :ab7413ab7468for HRP AEC :ab103742ab64252but it is only mentionned for IHC.Can I use these reagents for el...

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    Thank you very much for your enquiry. We do not currently sell substrates such as AP and HRP that have been tested in ELISPOT. This doesn’t mean that these products are not suitable, we just have not tested them in this application. The 4 products ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"