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AEC Substrate System (Ready to Use) (ab64252)


  • Product nameAEC Substrate System (Ready to Use)
  • Tested applicationsIHC-Pmore details


  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferConstituents: 0.03% Hydrogen peroxide, 3% DMF
    Note: stabilizers
  • Concentration information loading...
  • Reagent notes1. Prepare samples as required.
    2. Apply a sufficient volume of the AEC Single Solution reagent to cover the tissue section.
    3. Incubate the tissue section for a minimum of 10 minutes.
    4. Rinse slide in buffer.
    5. Apply counterstain according to manufacurer’s instructions.
    6. Apply a sufficient volume of an aqueous mounting medium to cover the section.
  • Research areas
  • RelevanceAEC (3-Amino-9-Ethylcarbazole) is a widely used chromogen for immunohistochemical staining. AEC produces a red end product that is soluble in alcohol and must be used with an aqueous counterstain and mounting media. AEC produces a clean, very intense stain that is free of unwanted background.
  • Alternative names
    • 3 amino 9 ethylcarbazole


Our Abpromise guarantee covers the use of ab64252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

AEC Substrate System (Ready to Use) images

  • Human colon carcinoma stained with anti-Keratin 20 antibody using AEC Substrate System (Ready to Use) (ab64252).

References for AEC Substrate System (Ready to Use) (ab64252)

ab64252 has not yet been referenced specifically in any publications.

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The kit will work with the rabbit and mouse primary antibodies.
You can try citrate buffer pH for a start and Tris/EDTA in case the staining does not work. Please check the following for protocol and troubleshooti...

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Heat mediated antigen retrieval method with microwave or pressure cooker is recommended for this ab.
The suitable detection system depends on the substrate you want to use. e.g. with DAB or AEC, we have following ...

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Yes, Mouse and Rabbit Specific HRP/DAB (ABC) detection IHC kit (ab64264) and AEC Substrate System (Ready to Use) (ab64252) can be used with rat sections.

Thank you very much for your enquiry. We do not currently sell substrates such as AP and HRP that have been tested in ELISPOT. This doesn’t mean that these products are not suitable, we just have not tested them in this application. The 4 produc...

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