Anti-AFF4 antibody (ab103586)
Key features and details
- Rabbit polyclonal to AFF4
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-AFF4 antibody
See all AFF4 primary antibodies -
Description
Rabbit polyclonal to AFF4 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein within Human AFF4 aa 1-400. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- Human and Mouse liver tissue lysates; AFF4 transfected 293T cell line lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab103586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 127 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 127 kDa. |
Target
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Relevance
May play a role in transcriptional regulation. Component of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor b (P-TEFb). -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 27125 Human
- Entrez Gene: 93736 Mouse
- Omim: 604417 Human
- SwissProt: Q9UHB7 Human
- SwissProt: Q9ESC8 Mouse
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Alternative names
- AF4/FMR2 family member 4 antibody
- AF5Q31 antibody
- Alf4 antibody
see all
Images
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Anti-AFF4 antibody (ab103586) at 1/500 dilution + Human liver tissue lysate at 50 µg
Predicted band size: 127 kDa -
Anti-AFF4 antibody (ab103586) at 1/500 dilution + Mouse liver tissue lysate at 50 µg
Predicted band size: 127 kDa -
All lanes : Anti-AFF4 antibody (ab103586) at 1/500 dilution
Lane 1 : AFF4 transfected 293T cell line lysate
Lane 2 : Non-transfected 293T cell line lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 127 kDa
Datasheets and documents
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Datasheet download
References (6)
ab103586 has been referenced in 6 publications.
- Li X et al. A proteolysis-targeting chimera molecule selectively degrades ENL and inhibits malignant gene expression and tumor growth. J Hematol Oncol 15:41 (2022). WB ; Mouse . PubMed: 35395864
- Wu F et al. Small-molecule inhibitor of AF9/ENL-DOT1L/AF4/AFF4 interactions suppresses malignant gene expression and tumor growth. Theranostics 11:8172-8184 (2021). PubMed: 34373735
- Li J et al. Long noncoding RNA ZFPM2-AS1 is involved in lung adenocarcinoma via miR-511-3p/AFF4 pathway. J Cell Biochem 121:2534-2542 (2020). PubMed: 31692047
- Zheng L et al. Triptonide Modulates MAPK Signaling Pathways and Exerts Anticancer Effects via ER Stress-Mediated Apoptosis Induction in Human Osteosarcoma Cells. Cancer Manag Res 12:5919-5929 (2020). PubMed: 32765093
- Olson CM et al. Pharmacological perturbation of CDK9 using selective CDK9 inhibition or degradation. Nat Chem Biol 14:163-170 (2018). PubMed: 29251720
- Zhou CC et al. AFF1 and AFF4 differentially regulate the osteogenic differentiation of human MSCs. Bone Res 5:17044 (2017). WB ; Human . PubMed: 28955517