|
|
|
|
Western blot - AFM antibody (ab49139)

(enlarge)
|
|
|
|
|
Product Name
|
AFM antibody
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to AFM
|
|
Immunogen
|
Synthetic peptide corresponding to a region of Human AFM
|
|
Reacts with
(species key)
|
Hu
|
|
Tested applications
(see key)
|
ELISA, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions ELISA: Use at an assay dependent dilution. (ELISA titre using peptide based assay: 1/12500) WB: Use at a concentration of 0.5 µg/ml. Predicted molecular weight: 69 kDa.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
HepG2 cell lysate
|
|
Cellular localization
|
Secreted
|
|
Research areas
|
Signal Transduction >> Metabolism >> Vitamins / Minerals Cardiovascular >> Blood >> Serum Proteins
|
|
Relevance
|
AFM (afamin) is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These genes encode structurally related serum transport proteins that are thought to be evolutionarily related. AFM is regulated developmentally, expressed in the liver and secreted into the bloodstream
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window
|
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: None Constituents: 2% Sucrose, PBS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this AFM antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for AFM antibody images, references, products related to ab49139 and other tools.
|
| |
AFM antibody images:
|
Western blot - AFM antibody (ab49139)
All lanes : AFM antibody (ab49139) at 0.5 µg/ml
Lane 1 : Molecular weight marker Lane 2 : HepG2 cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50,000 developed using the ECL technique
Predicted band size : 69 kDa Observed band size : 69 kDa
Gel concentration: 12%
|
| |
| |
|
Search PubMed (MEDLINE) for references to AFM
|
| |
AFM antibody - more information
|
Customer reviews (feedback) regarding AFM antibody |
Customer FAQs regarding AFM antibody |
Protocols for AFM antibody |
Price and availability of products related to AFM antibody |
| |
AFM antibody - related products:
|
|
|
| |
AFM antibody - other tools:
|
Contact Abcam with a Technical Enquiry about AFM antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"