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Products:Cardiovascular >> Blood >> Serum Proteins
Overview
Product name
Anti-AFM antibody
See all AFM products (2) ...
Description
Rabbit polyclonal to AFM
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
A region within synthetic peptide: GQCIINSNKD DRPKDLSLRE GKFTDSENVC QERDADPDTF FAKFTFEYSR, corresponding to amino acids 311-360 of Human AFM
GQCIINSNKD DRPKDLSLRE GKFTDSENVC QERDADPDTF FAKFTFEYSR
Positive control
HepG2 cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Metabolism >> Vitamins / Minerals
Cardiovascular >> Blood >> Serum Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab49139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 µg/mlPredicted molecular weight: 69 kDa.
IHC-P: Use a concentration of 5 µg/mlPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Target
Function
Vitamin E binding protein. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient or across the blood-brain barrier.
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains.
Post-translational
modifications
N-glycosylated.
Cellular localization
Secreted.
Target information above from: UniProt accessionP43652
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AFAM_HUMAN antibody
- Afamin antibody
- Afm antibody
see all
Database links
- Entrez Gene: 173 Human
- Omim: 104145 Human
- SwissProt: P43652 Human
- Unigene: 168718 Human
Anti-AFM antibody images:
Western blot - AFM antibody (ab49139)
All lanes : Anti-AFM antibody (ab49139) at 0.5 µg/ml
Lane 1 : Molecular weight marker
Lane 2 : HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50,000
developed using the ECL technique
Predicted band size : 69 kDa
Observed band size : 69 kDa
Gel concentration: 12%
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AFM antibody (ab49139)
IHC image of ab49139 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab49139, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-AFM antibody (ab49139)
ab49139 has not yet been referenced specifically in any publications.
Publishing research using ab49139? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"