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Western blot - AFM antibody (ab49139)
Western blot - AFM antibody (ab49139)
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Product Name 

AFM antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to AFM

Immunogen 

Synthetic peptide corresponding to a region of Human AFM

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
(ELISA titre using peptide based assay: 1/12500)
WB: Use at a concentration of 0.5 µg/ml. Predicted molecular weight: 69 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HepG2 cell lysate

Cellular localization 

Secreted

Research areas 

Signal Transduction >> Metabolism >> Vitamins / Minerals
Cardiovascular >> Blood >> Serum Proteins

Relevance 

AFM (afamin) is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These genes encode structurally related serum transport proteins that are thought to be evolutionarily related. AFM is regulated developmentally, expressed in the liver and secreted into the bloodstream

 

Alternative names AFM antibody (ab49139)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

173    (Human)

Omim

    

104145    (Human)

SwissProt

    

P43652    (Human)

Unigene

    

168718    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this AFM antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for AFM antibody images, references, products related to ab49139 and other tools.

 

AFM antibody images:

Product image  Western blot - AFM antibody (ab49139)

Western blot - AFM antibody (ab49139)

All lanes : AFM antibody (ab49139) at 0.5 µg/ml

Lane 1 : Molecular weight marker
Lane 2 : HepG2 cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50,000
developed using the ECL technique

Predicted band size : 69 kDa
Observed band size : 69 kDa


Gel concentration: 12%

 
 

Search PubMed (MEDLINE) for references to AFM

 

AFM antibody - more information

Customer reviews (feedback) regarding AFM antibody

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Price and availability of products related to AFM antibody

 

AFM antibody - related products:

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