Anti-AGAP1 antibody (ab151480)
Key features and details
- Rabbit polyclonal to AGAP1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AGAP1 antibody
See all AGAP1 primary antibodies -
Description
Rabbit polyclonal to AGAP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Xenopus laevis -
Immunogen
Recombinant fragment corresponding to Human AGAP1 aa 1-300.
Database link: Q9UPQ3 -
Positive control
- MCF7 whole cell lysate; MDAMB231 xenograft tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 45 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 45 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
GTPase-activating protein for ARF1 and, to a lesser extent, ARF5. Directly and specifically regulates the adapter protein 3 (AP-3)-dependent trafficking of proteins in the endosomal-lysosomal system. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the centaurin gamma-like family.
Contains 2 ANK repeats.
Contains 1 Arf-GAP domain.
Contains 1 PH domain. -
Domain
The PH domain mediates AP-3 binding. -
Post-translational
modificationsPhosphorylated on tyrosines. -
Cellular localization
Cytoplasm. Associates with the endocytic compartment. - Information by UniProt
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Database links
- Entrez Gene: 116987 Human
- Entrez Gene: 347722 Mouse
- Entrez Gene: 431967 Xenopus laevis
- Omim: 608651 Human
- SwissProt: Q9UPQ3 Human
- SwissProt: Q8BXK8 Mouse
- SwissProt: Q6NRL1 Xenopus laevis
- Unigene: 435039 Human
see all -
Alternative names
- AGAP 1 antibody
- AGAP-1 antibody
- Agap1 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab151480 has been referenced in 1 publication.
- Cheng N et al. Crystal structure of the GTP-binding protein-like domain of AGAP1. Acta Crystallogr F Struct Biol Commun 77:105-112 (2021). PubMed: 33830075