Recombinant
RabMAb

Anti-AGL antibody [EPR8880] (Alexa Fluor® 488) (ab216381)

Overview

  • Product name
    Anti-AGL antibody [EPR8880] (Alexa Fluor® 488)
    See all AGL primary antibodies
  • Description
    Rabbit monoclonal [EPR8880] to AGL (Alexa Fluor® 488)
  • Host species
    Rabbit
  • Conjugation
    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications
    Suitable for: ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human AGL aa 50-150. The exact sequence is proprietary.
    Database link: EPR8880

  • Positive control
    • ICC/IF: HeLa cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab216381 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)

Target

  • Function
    Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase in glycogen degradation.
  • Tissue specificity
    Liver, kidney and lymphoblastoid cells express predominantly isoform 1; whereas muscle and heart express not only isoform 1, but also muscle-specific isoform mRNAs (isoforms 2, 3 and 4). Isoforms 5 and 6 are present in both liver and muscle.
  • Involvement in disease
    Glycogen storage disease 3
  • Sequence similarities
    Belongs to the glycogen debranching enzyme family.
  • Post-translational
    modifications
    The N-terminus is blocked.
    Ubiquitinated.
  • Cellular localization
    Cytoplasm. Under glycogenolytic conditions localizes to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1110061O17Rik antibody
    • 4-1 antibody
    • 4-glucantransferase antibody
    • 6-glucosidase antibody
    • 9430004C13Rik antibody
    • 9630046L06Rik antibody
    • AGL antibody
    • AI850929 antibody
    • Amylo 1 6 glucosidase 4 alpha glucanotransferase antibody
    • Amylo-1 antibody
    • Amylo-alpha-1 antibody
    • C77197 antibody
    • Dextrin 6-alpha-D-glucosidase antibody
    • GDE antibody
    • GDE_HUMAN antibody
    • Glycogen debrancher antibody
    • Glycogen debranching enzyme antibody
    • Glycogen storage disease type III antibody
    • Oligo-1 antibody
    see all

Images

  • ab216381 staining AGL in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab216381 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab216381 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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