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Anti-AGXT antibody [1F9BC7] (ab110313)

MSCatalog No. MS773

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Overview

Product name

Anti-AGXT antibody [1F9BC7]
See all AGXT products (3) ...

Description

Mouse monoclonal [1F9BC7] to AGXT

Tested applications

ICC/IF, IP, In-Cell ELISAmore details

Cross reactivity

Reacts with

Rat, Cow, Human

Immunogen

Human liver mitochondria

Positive control

HepG2 cells; Human and Rat liver, Bovine heart and HepG2 cell lysates.

Properties

Form

Liquid

Storage instructions

Store at +4°C. Do not freeze.

Storage buffer

Preservative: 0.02% Sodium azide
Constituent: HBS

Concentration

Concentration information loading...

Purity

>95% by SDS-PAGE

Purification notes

ab110313 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Clonality

Monoclonal

Clone number

1F9BC7

Isotype

IgG1

Light chain type

kappa

Research areas

Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Energy Metabolism
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Amino acid metabolism
Signal Transduction >> Metabolism >> Energy Metabolism
Signal Transduction >> Metabolism >> Amino Acids

  • Immunocytochemistry/ Immunofluorescence - Anti-AGXT antibody [1F9BC7] (ab110313)Immunocytochemistry/ Immunofluorescence - Anti-AGXT antibody [1F9BC7] (ab110313) image (enlarge)

  • Immunoprecipitation - Anti-AGXT antibody [1F9BC7] (ab110313)Immunoprecipitation - Anti-AGXT antibody [1F9BC7] (ab110313) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab110313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Tissue specificity

Liver.

Involvement in disease

Defects in AGXT are the cause of hyperoxaluria primary type 1 (HP1) [MIM:259900]; also known as primary hyperoxaluria type I (PH1) and oxalosis I. HP1 is a rare autosomal recessive inborn error of glyoxylate metabolism characterized by increased excretion of oxalate and glycolate, and the progressive accumulation of insoluble calcium oxalate in the kidney and urinary tract.

Sequence similarities

Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.

Cellular localization

Peroxisome. Mitochondrion matrix. Except in some HP1 patients where AGT is found in the mitochondrial matrix.

Target information above from: UniProt accessionP21549 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • AGT antibody
  • AGT1 antibody
  • Agxt antibody
  • AGXT1 antibody
  • Alanine glyoxylate aminotransferase antibody
  • Alanine glyoxylate aminotransferase3 antibody
  • Alanine--glyoxylate aminotransferase antibody
  • EC 2.6.1.44 antibody
  • EC 2.6.1.51 antibody
  • Hepatic peroxisomal alanine glyoxylate aminotransferase antibody
  • L alanine glyoxylate aminotransferase 1 antibody
  • MS773 antibody
  • PH1 antibody
  • Serine pyruvate aminotransferase antibody
  • Serine--pyruvate aminotransferase antibody
  • Serine--pyruvate aminotransferase, mitochondrial antibody
  • SPAT antibody
  • SPT antibody
  • SPYA_HUMAN antibody
  • TLH6 antibody
see all

Database links

Anti-AGXT antibody [1F9BC7] images:

  Immunocytochemistry/ Immunofluorescence - Anti-AGXT antibody [1F9BC7] (ab110313)

Immunocytochemistry/ Immunofluorescence - Anti-AGXT antibody [1F9BC7] (ab110313)

Immunocytochemistry analysis using ab110313 at 5 ug/ml staining AGXT in HepG2 cells (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (green).

  Immunoprecipitation - Anti-AGXT antibody [1F9BC7] (ab110313)

Immunoprecipitation - Anti-AGXT antibody [1F9BC7] (ab110313)

Using MitoSciences' standard immunoprecipitation protocol, ab110313 precipicates with the 43kDa AGXT protein in human liver samples. Very small amounts of AGXT are immunoprecipitated from bovine heart, rat liver or human HepG2 cultured cell lysate. Identity of this protein was confirmed by mass spectrometry. This gel was stained with silver.

References for Anti-AGXT antibody [1F9BC7] (ab110313)

ab110313 has not yet been referenced specifically in any publications.

Publishing research using ab110313? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"