Anti-AHA1 antibody [25F2.D9] (ab91259)

Overview

• Product nameAnti-AHA1 antibody [25F2.D9]See all AHA1 primary antibodies ...
• Description
  Rat monoclonal [25F2.D9] to AHA1
• Tested applicationsWB, IP, ELISA, IHC-P, IHC-Fr, ICC, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Mouse AHA1

• Positive controlRat tissue lysate

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone number25F2.D9
• IsotypeIgG2a
• Light chain typekappa
• Research Areas
  • Signal Transduction
  • Protein Trafficking
  • Chaperones
  • Heat Shock Proteins

Applications

Target

• FunctionCochaperone that stimulates HSP90 ATPase activity (By similarity). May affect a step in the endoplasmic reticulum to Golgi trafficking.
• Tissue specificityExpressed in numerous tissues, including brain, heart, skeletal muscle and kidney and, at lower levels, liver and placenta.
• Sequence similaritiesBelongs to the AHA1 family.
• Cellular localizationCytoplasm > cytosol. Endoplasmic reticulum. May transiently interact with the endoplasmic reticulum.

Target information above from: UniProt accession O95433 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 10598 Human
  • Entrez Gene: 217737 Mouse
  • Omim: 608466 Human
  • SwissProt: O95433 Human
  • SwissProt: Q8BK64 Mouse
  • Unigene: 204041 Human
  • Unigene: 22626 Mouse

• Alternative names
  Activator of 90 kDa heat shock protein ATPase homolog 1 antibodyActivator of heat shock 90kDa protein ATPase homolog 1 antibodyAHA 1 antibody

  AHA1 antibodyAHSA 1 antibodyAHSA1 antibodyAHSA1_HUMAN antibodyC14orf3 antibodyHSPC322 antibodyp38 antibody

  see all

Anti-AHA1 antibody [25F2.D9] images

• 

  Immunocytochemistry/ Immunofluorescence - AHA1 antibody [25F2.D9] (ab91259)
  ab91259 staining AHA1 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluoresence). Cells were fixed with 4% PFA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 1 hour at room temperature. A Fluoroscein-conjugated goat anti-rat IgG secondary antibody was used.
• 

  Immunoprecipitation - AHA1 antibody [25F2.D9] (ab91259)
  ab91259 used in the Immunoprecipitation of AHA1 from Human Hela cells. The antibody was used at 20µl per 1 ml of cell lysate (50mM Tris (pH 7.2); 150mM NaCl; 1mM MgCl₂; 1% TX-100; protease inhibitors); captured on 20µl of protein G beads .