Overview

  • Product nameAnti-AHA1 antibodySee all AHA1 primary antibodies ...
  • Description
    Rabbit polyclonal to AHA1
  • Tested applicationsICC/IF, IHC-P, IHC-Fr, WB, ELISA, IP, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Full length protein (Mouse)

  • Positive control
    • This antibody gave a positive result when used in the following formaldehyde fixed cell lines: PC3.

Properties

Applications

Our Abpromise guarantee covers the use of ab83036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
IHC-P 1/10000.
IHC-Fr 1/10000.
WB 1/1000 - 1/2000. Predicted molecular weight: 38 kDa.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

Target

  • FunctionCochaperone that stimulates HSP90 ATPase activity (By similarity). May affect a step in the endoplasmic reticulum to Golgi trafficking.
  • Tissue specificityExpressed in numerous tissues, including brain, heart, skeletal muscle and kidney and, at lower levels, liver and placenta.
  • Sequence similaritiesBelongs to the AHA1 family.
  • Cellular localizationCytoplasm > cytosol. Endoplasmic reticulum. May transiently interact with the endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activator of 90 kDa heat shock protein ATPase homolog 1 antibody
    • Activator of heat shock 90kDa protein ATPase homolog 1 antibody
    • AHA 1 antibody
    • AHA1 antibody
    • AHSA 1 antibody
    • AHSA1 antibody
    • AHSA1_HUMAN antibody
    • C14orf3 antibody
    • HSPC322 antibody
    • p38 antibody
    see all

Anti-AHA1 antibody images

  • ab83036 stained PC3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab83036 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-AHA1 antibody (ab83036)

    Lane 1 : Cell lysates prepared from A431 cells
    Lane 2 : Cell lysates prepared from A549 cells
    Lane 3 : Cell lysates prepared from HCT116 cells
    Lane 4 : Cell lysate prepared from Hela cells
    Lane 5 : Cell lysate prepared from HEK293 cells
    Lane 6 : Cell lysates prepared from HepG2 cells
    Lane 7 : Cell lysates prepared from HL-60 cells
    Lane 8 : Cell lysates prepared from HUVEC cells
    Lane 9 : Cell lysates prepared from Jurkat cells
    Lane 10 : Cell lysates prepared from MCF7 cells
    Lane 11 : Cell lysates prepared from PC3 cells
    Lane 12 : Cell lysates prepared from T98G cells
    Lane 13 : Lysate prepared from rat brain

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 38 kDa

References for Anti-AHA1 antibody (ab83036)

This product has been referenced in:
  • Moura CS  et al. Whey protein hydrolysate enhances HSP90 but does not alter HSP60 and HSP25 in skeletal muscle of rats. PLoS One 9:e83437 (2014). WB ; Rat . Read more (PubMed: 24465380) »

See 1 Publication for this product

Product Wall

Thank you for contacting us. We have 4 anti-AHA1 antibodies: ab91259, ab83036, ab56721 and ab64998. The first 3 antibodies were raised against the full length mouse or human protein, respectively. The sequence homology with these ...

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Thank you for contacting us. Unfortunately, none of the antibodies you mentioned (ab91259, ab83036, ab13492) have been tested in zebrafish so far. However, we have another HSP90 antibody that was tested in zebrafish: ab13494 (http://w...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"