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Read our guarantee »Anti-AHRR antibody
See all AHRR products (3) ...
Mouse polyclonal to AHRR
Reacts with
Human
Recombinant fragment: YISHLGHGVR GAQPHGRATA GRSRELTPFH PAHCACLEPT DGLPQSEPPH QLCARGRGEQ SCTCRAAEAA PVVKREPLDS PQWATHSQGM VPGMLPKSAL , corresponding to internal sequence amino acids 601-700 of Human AHRR
YISHLGHGVR GAQPHGRATA GRSRELTPFH PAHCACLEPT DGLPQSEPPH QLCARGRGEQ SCTCRAAEAA PVVKREPLDS PQWATHSQGM VPGMLPKSAL
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: None
Constituents: 50% Glycerol, Whole serum
Whole antiserum
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
Polyclonal
IgG
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> NFkB pathway
Our Abpromise guarantee covers the use of ab43199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000. Predicted molecular weight: 78 kDa.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Mediates dioxin toxicity and is involved in regulation of cell growth and differentiation. Represses the transcription activity of AHR by competing with this transcription factor for heterodimer formation with the ARNT and subsequently binding to the xenobiotic response element (XRE) sequence present in the promoter regulatory region of variety of genes. Represses CYP1A1 by binding the XRE sequence and recruiting ANKRA2, HDAC4 and/or HDAC5. Autoregulates its expression by associating with its own XRE site.
Highly expressed in testis, lung, ovary, spleen and pancreas. Highly expressed in mononuclear cells (MNCs) from umbilical cord blood. Isoform 3 is highly expressed in lung, kidney, spleen and thymus. Down-regulated malignant tissue from different anatomical origins, including colon, breast, lung, stomach, cervix, and ovary.
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAS (PER-ARNT-SIM) domain.
Cytoplasm. Nucleus. Predominantly in the nuclear compartment. First cytoplasmic, translocates into the nuclear compartment upon interaction with ARNT in the cytoplasmic compartment.
Target information above from: UniProt accessionA9YTQ3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
ab43199 has not yet been referenced specifically in any publications.
Publishing research using ab43199? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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