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Product Name
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AHRR antibody
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See all AHRR antibodies (2)...
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to AHRR
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Immunogen
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Recombinant fragment: YISHLGHGVR GAQPHGRATA GRSRELTPFH PAHCACLEPT DGLPQSEPPH QLCARGRGEQ SCTCRAAEAA PVVKREPLDS PQWATHSQGM VPGMLPKSAL , corresponding to internal sequence amino acids 601-700 of Human AHRR BLAST 'YISHLGHGVR GAQPHGRATA GRSRELTPFH PAHCACLEPT DGLPQSEPPH QLCARGRGEQ SCTCRAAEAA PVVKREPLDS PQWATHSQGM VPGMLPKSAL ' with or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions WB: 1/1000. Predicted molecular weight: 78 kDa.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> NFkB pathway
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Relevance
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AHRR contains 1 basic helix-loop-helix (bHLH) domain. It represses signal transduction by the arylhydrocarbon receptor by competing with the arylhydrocarbon receptor nuclear translocator for binding to the arylhydrocarbon receptor. This interaction is part of a negative feedback regulatory mechanism. AHRR is involved in the teratogenic effects of dioxin. In addition, the encoded protein can bind to nuclear factor kappa B.
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: None Constituents: 50% Glycerol, Whole serum Material safety datasheet (MSDS) for this product: Glycerol MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this AHRR antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to AHRR
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AHRR antibody - more information
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Customer reviews (feedback) regarding AHRR antibody |
Customer FAQs regarding AHRR antibody |
Protocols for AHRR antibody |
Price and availability of products related to AHRR antibody |
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