AHRR antibody (ab43199)

Product Name 

AHRR antibody

Product type 

Primary antibodies

Description 

Mouse polyclonal to AHRR

Immunogen 

Recombinant fragment: YISHLGHGVR GAQPHGRATA GRSRELTPFH PAHCACLEPT DGLPQSEPPH QLCARGRGEQ SCTCRAAEAA PVVKREPLDS PQWATHSQGM VPGMLPKSAL , corresponding to internal sequence amino acids 601-700 of Human AHRR
BLAST 'YISHLGHGVR GAQPHGRATA GRSRELTPFH PAHCACLEPT DGLPQSEPPH QLCARGRGEQ SCTCRAAEAA PVVKREPLDS PQWATHSQGM VPGMLPKSAL ' with or

Reacts with 
(species key)

Hu

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
WB: 1/1000. Predicted molecular weight: 78 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic and Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> NFkB pathway

Relevance 

AHRR contains 1 basic helix-loop-helix (bHLH) domain. It represses signal transduction by the arylhydrocarbon receptor by competing with the arylhydrocarbon receptor nuclear translocator for binding to the arylhydrocarbon receptor. This interaction is part of a negative feedback regulatory mechanism. AHRR is involved in the teratogenic effects of dioxin. In addition, the encoded protein can bind to nuclear factor kappa B.

This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Database links 

Raised in 

Mouse

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: None
Constituents: 50% Glycerol, Whole serum

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Customer reviews (feedback) regarding AHRR antibody

Customer FAQs regarding AHRR antibody

Protocols for AHRR antibody

Price and availability of products related to AHRR antibody

AHRR antibody - related products: