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Overview
Product name
Anti-AHRR antibody
See all AHRR products (3) ...
Description
Rabbit polyclonal to AHRR
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide corresponding to a region within internal sequence amino acids 648-697 (PQSEPPHQLC ARGRGEQSCT CRAAEAAPVV KREPLDSPQW ATHSQGMVPG) of Human AHRR (NP_065782)
PQSEPPHQLC ARGRGEQSCT CRAAEAAPVV KREPLDSPQW ATHSQGMVPG
Positive control
HepG2 cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> NFkB pathway
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Western blot - AHRR antibody (ab85666)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab85666 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 78 kDa.
Target
Function
Mediates dioxin toxicity and is involved in regulation of cell growth and differentiation. Represses the transcription activity of AHR by competing with this transcription factor for heterodimer formation with the ARNT and subsequently binding to the xenobiotic response element (XRE) sequence present in the promoter regulatory region of variety of genes. Represses CYP1A1 by binding the XRE sequence and recruiting ANKRA2, HDAC4 and/or HDAC5. Autoregulates its expression by associating with its own XRE site.
Tissue specificity
Highly expressed in testis, lung, ovary, spleen and pancreas. Highly expressed in mononuclear cells (MNCs) from umbilical cord blood. Isoform 3 is highly expressed in lung, kidney, spleen and thymus. Down-regulated malignant tissue from different anatomical origins, including colon, breast, lung, stomach, cervix, and ovary.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAS (PER-ARNT-SIM) domain.
Cellular localization
Cytoplasm. Nucleus. Predominantly in the nuclear compartment. First cytoplasmic, translocates into the nuclear compartment upon interaction with ARNT in the cytoplasmic compartment.
Target information above from: UniProt accessionA9YTQ3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AHH antibody
- AhR repressor antibody
- AhRR antibody
see all
Database links
- Entrez Gene: 57491 Human
- Entrez Gene: 11624 Mouse
- Entrez Gene: 498999 Rat
- Omim: 606517 Human
- SwissProt: A9YTQ3 Human
- SwissProt: Q3U1U7 Mouse
- SwissProt: Q75NT5 Rat
- Unigene: 50823 Human
see all
Anti-AHRR antibody images:
Western blot - AHRR antibody (ab85666)
Anti-AHRR antibody (ab85666) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 78 kDa
Observed band size : 92 kDa (why is the actual band size different from the predicted?)
Gel concentration 12%
References for Anti-AHRR antibody (ab85666)
This product has been referenced in:
- To KKet al. Upregulation of ABCG2 by Romidepsin via the Aryl Hydrocarbon Receptor Pathway. Mol Cancer Res 9:516-27 (2011). WB; Human.Read more (PubMed: 21357443) »
See 1 publication for this product
Publishing research using ab85666? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"