AIBZIP 293T Transfected Lysate - (positive control) (ab94227)

Overview

  • Product nameAIBZIP 293T Transfected Lysate - (positive control)
  • General notesab94227 is a 293T cell transfected lysate in which Human AIBZIP has been transiently over-expressed using a pCMV-AIBZIP plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsWBmore details

Properties

  • BackgroundFunction: Transcriptional activator that may play a role in the unfolded protein response. Binds to the UPR element (UPRE) but not to CRE element. Preferentially binds DNA with to the consensus sequence 5'-T[GT]ACGT[GA][GT]-3' and has transcriptional activation activity from UPRE. Binds to NF-kappa-B site and has transcriptional activation activity from NF-kappa-B-containing regulatory elements. Tissue specificity: According to PubMed:11830526, exclusively expressed in the prostate. Expressed in breast and prostate cancer cell lines. Expressed in prostatic luminal epithelial cells (at protein level). Expression is significantly more abundant in prostate cancer than in benign prostatic tissue (prostatic hyperplasia). According to PubMed:12111373, also expressed in brain, pancreas and skeletal muscle, and at lower levels in small intestine, testis, leukocyte and thymus. Similarity: Belongs to the bZIP family. ATF subfamily. Contains 1 bZIP domain. PTM: N-glycosylated in the C-terminal region. Controlled by regulated intramembrane proteolysis (RIP). Following ER stress a fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases (PS1 and PS2). PS1 cleavage may be suppressed by a determinant in the C-terminal region.

Applications

Our Abpromise guarantee covers the use of ab94227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • AIBZIP 293T Transfected Lysate - (positive control) images

    • ab94227 at 15µg/lane on an SDS-PAGE gel
    • All lanes : Anti-AIBZIP antibody (ab58366) at 1/500 dilution

      Lane 1 : AIBZIP 293T Transfected Lysate - (positive control) (ab94227)
      Lane 2 : 293T Non Transfected Lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for AIBZIP 293T Transfected Lysate - (positive control) (ab94227)

    ab94227 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"