Overview
- Product nameAnti-AICDA antibody - ChIP GradeSee all AICDA primary antibodies ...
- DescriptionRabbit polyclonal to AICDA - ChIP Grade
- Tested applicationsChIP, IHC-P, WB, ICC more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse - Immunogen
Synthetic peptide corresponding to 14 amino acids near the C-terminus of Human AID
- Positive controlRamos cells
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1X PBS -
Concentration information loading... - PurityIgG fraction
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab59361 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ChIP | ChIP: Use at an assay dependent dilution. |
| IHC-P | IHC-P: Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
| WB | WB: Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa). |
| ICC | ICC: Use a concentration of 10 µg/ml. |
Target
- FunctionRNA-editing deaminase involved in somatic hypermutation, gene conversion, and class-switch recombination. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses.
- Tissue specificityStrongly expressed in lymph nodes and tonsils.
- Involvement in diseaseDefects in AICDA are the cause of hyper-IgM immunodeficiency syndrome type 2 (HIGM2) [MIM:605258]; also known as hyper-IgM syndrome 2. HIGM2 is an autosomal recessive disorder characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE, resulting in a profound susceptibility to bacterial infections. HIGM2 causes the absence of Ig class switch recombination (CSR), the lack of Ig somatic hypermutations, and lymph node hyperplasia caused by the presence of giant germinal centers.
- Sequence similaritiesBelongs to the cytidine and deoxycytidylate deaminase family.
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Database links
- Entrez Gene: 57379 Human
- Entrez Gene: 11628 Mouse
- Omim: 605257 Human
- SwissProt: Q9GZX7 Human
- SwissProt: Q9WVE0 Mouse
- Unigene: 149342 Human
- Unigene: 391503 Mouse
Target information above from: UniProt accession
Q9GZX7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Activation induced cytidine deaminase antibodyActivation induced deaminase antibodyActivation-induced cytidine deaminase antibody
- AICDA antibodyAICDA_HUMAN antibodyAID antibodyARP 2 antibodyARP2 antibodyCDA 2 antibodyCDA2 antibodyCytidine aminohydrolase antibodyHIGM2 antibodyIntegrated into Burkitt's lymphoma cell line Ramos antibody
see all
Anti-AICDA antibody - ChIP Grade images
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Western blot - AID antibody (ab59361)All lanes : Anti-AICDA antibody - ChIP Grade (ab59361) at 2 µg/ml
Lane 1 : Ramos cell lysate
Lane 2 : Ramos cell lysate with immunizing peptide
Lysates/proteins at 15 µg per lane.
Predicted band size : 24 kDa
Observed band size : 24 kDa -
Immunocytochemistry - AID antibody (ab59361)ab59361 at 10µg/ml, staining human AID in Ramos cells -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICDA antibody - ChIP Grade (ab59361)ab59361 (4 µg/ml) staining AICDA in human tonsil using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic staining.
Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
References for Anti-AICDA antibody - ChIP Grade (ab59361)
This product has been referenced in:
- Geisberger R et al. Lysine residue at position 22 of the AID protein regulates its class switch activity. PLoS One 7:e30667 (2012). WB . Read more (PubMed: 22363466) »
- Häsler J et al. Cytoplasmic activation-induced cytidine deaminase (AID) exists in stoichiometric complex with translation elongation factor 1a (eEF1A). Proc Natl Acad Sci U S A 108:18366-71 (2011). WB ; Human . Read more (PubMed: 22042842) »
