Anti-AICDA antibody (ab183618)
Key features and details
- Rabbit polyclonal to AICDA
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-AICDA antibody
See all AICDA primary antibodies -
Description
Rabbit polyclonal to AICDA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee -
Immunogen
Recombinant fragment within Human AICDA aa 67-198. The exact sequence is proprietary.
Database link: Q9GZX7 -
Positive control
- K562, THP1 and HL60 whole cell lysates; SAS xenograft.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 23 kDa.
|
|
IHC-P |
1/100 - 1/1000.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 23 kDa. |
IHC-P
1/100 - 1/1000. |
Target
-
Function
RNA-editing deaminase involved in somatic hypermutation, gene conversion, and class-switch recombination. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. -
Tissue specificity
Strongly expressed in lymph nodes and tonsils. -
Involvement in disease
Defects in AICDA are the cause of hyper-IgM immunodeficiency syndrome type 2 (HIGM2) [MIM:605258]; also known as hyper-IgM syndrome 2. HIGM2 is an autosomal recessive disorder characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE, resulting in a profound susceptibility to bacterial infections. HIGM2 causes the absence of Ig class switch recombination (CSR), the lack of Ig somatic hypermutations, and lymph node hyperplasia caused by the presence of giant germinal centers. -
Sequence similarities
Belongs to the cytidine and deoxycytidylate deaminase family. - Information by UniProt
-
Database links
- Entrez Gene: 473404 Chimpanzee
- Entrez Gene: 57379 Human
- Omim: 605257 Human
- SwissProt: Q9GZX7 Human
- Unigene: 149342 Human
-
Alternative names
- Activation induced cytidine deaminase antibody
- Activation induced deaminase antibody
- Activation-induced cytidine deaminase antibody
see all
Images
-
All lanes : Anti-AICDA antibody (ab183618) at 1/1000 dilution
Lane 1 : K562 whole cell lysate
Lane 2 : THP1 whole cell lysate
Lane 3 : HL60 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 23 kDa12% SDS PAGE
-
Immunohistochemical analysis of paraffin embedded SAS xenograft labeling AICDA with ab183618 at 1/500 dilution.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab183618 has not yet been referenced specifically in any publications.