Loading...
|
ab13718 |
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Products:Immunology >> Immunoglobulins >> Class Switching
Anti-AICDA antibody
See all AICDA products (8) ...
Rabbit polyclonal to AICDA
Reacts with
Human
Predicted to work with
Mouse
Synthetic peptide derived from residues 1 - 100 of Human AID.
.
This antibody gave a positive signal in AICDA 293T Cell Transient Overexpression Lysate.
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
Immunology >> Immunoglobulins >> Class Switching
Western blot - AICDA antibody (ab5197)
(enlarge)
Our Abpromise guarantee covers the use of ab5197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
RNA-editing deaminase involved in somatic hypermutation, gene conversion, and class-switch recombination. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses.
Strongly expressed in lymph nodes and tonsils.
Defects in AICDA are the cause of hyper-IgM immunodeficiency syndrome type 2 (HIGM2) [MIM:605258]; also known as hyper-IgM syndrome 2. HIGM2 is an autosomal recessive disorder characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE, resulting in a profound susceptibility to bacterial infections. HIGM2 causes the absence of Ig class switch recombination (CSR), the lack of Ig somatic hypermutations, and lymph node hyperplasia caused by the presence of giant germinal centers.
Belongs to the cytidine and deoxycytidylate deaminase family.
Target information above from: UniProt accessionQ9GZX7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - AICDA antibody (ab5197)

Anti-AICDA antibody (ab5197) at 1 µg/ml + AICDA 293T Cell Transient Overexpression Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
Predicted band size : 24 kDa
Observed band size : 24 kDa
This product has been referenced in:
See 1 publication for this product
Publishing research using ab5197? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:

Anti-AICDA antibody (ab5197) at 1 µg/ml + AICDA 293T Cell Transient Overexpression Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
Predicted band size : 24 kDa
Observed band size : 24 kDa
0
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Call 01223 696 000 or contact us
