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Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab5197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).Can be blocked with AID peptide (ab13718).
ChIP ChIP: Use at an assay dependent concentration.

Target

  • FunctionRNA-editing deaminase involved in somatic hypermutation, gene conversion, and class-switch recombination. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses.
  • Tissue specificityStrongly expressed in lymph nodes and tonsils.
  • Involvement in diseaseDefects in AICDA are the cause of hyper-IgM immunodeficiency syndrome type 2 (HIGM2) [MIM:605258]; also known as hyper-IgM syndrome 2. HIGM2 is an autosomal recessive disorder characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE, resulting in a profound susceptibility to bacterial infections. HIGM2 causes the absence of Ig class switch recombination (CSR), the lack of Ig somatic hypermutations, and lymph node hyperplasia caused by the presence of giant germinal centers.
  • Sequence similaritiesBelongs to the cytidine and deoxycytidylate deaminase family.

    • Target information above from: UniProt accession Q9GZX7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Activation induced cytidine deaminase antibodyActivation induced deaminase antibodyActivation-Induced Cytidine Deaminase antibody
        AICD antibodyAICDA antibodyAICDA_HUMAN antibodyAID antibodyARP 2 antibodyARP2 antibodyCDA 2 antibodyCDA2 antibodyCytidine aminohydrolase antibodyHIGM2 antibodyIntegrated into Burkitt's lymphoma cell line Ramos antibody
      see all

    Anti-AICDA antibody images

    • Western blot - AICDA antibody (ab5197)
      Western blot - AICDA antibody (ab5197)
      Anti-AICDA antibody (ab5197) at 1 µg/ml + AICDA 293T Cell Transient Overexpression Lysate at 10 µg

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution

      Predicted band size : 24 kDa
      Observed band size : 24 kDa

    References for Anti-AICDA antibody (ab5197)

    This product has been referenced in:
    • Dayal S  et al. High resolution analysis of the chromatin landscape of the IgE switch region in human B cells. PLoS One 6:e24571 (2011). ChIP ; Human . Read more (PubMed: 21949728) »
    • Duquette ML  et al. AID binds to transcription-induced structures in c-MYC that map to regions associated with translocation and hypermutation. Oncogene 24:5791-8 (2005). Read more (PubMed: 15940261) »

    See all 2 Publications for this product

    Publishing research using ab5197 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab5197 Submit a Question for ab5197

    Displaying 1 - 5 of 5 results for Abreviews and Q&A

    10
    Votes: Highest First

    Would like to try this antibody in flow cytometry.

    DISCOUNT CODE: ***
    Expiration date: February 13, 2013
    Value: $337

    This code will give you $337 off your next order before the expiration date. To redeem this offer, please submit an Abreview for ab5197 in flow cytometry and include thi...

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    ChIP abreview for Anti-AICDA antibody

     Good
    Abreviews
    Application ChIP
    Sample Human Cell lysate - other (Primary tonsillar B cells)
    Specification Primary tonsillar B cells
    Type Cross-linking (X-ChIP)
    Duration of cross-linking step: 4 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% Formaldehyde
    Detection step Taqman
    Positive control AID ChIP followed by qPCR analysis for known AID binding sites:
    IgG switch region
    IgM switch region
    BIRC3 promoter
    Negative control AID ChIP followed by qPCR analysis for known sites where AID is known not to bind:
    Neuro D1 gene
    Read More
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    Verified customer

    Submitted Aug 13 2012

    Phone call requesting further information on Anti-AICDA antibody (ab5197)

    Thank you for contacting us this morning.

    As discussed over the phone, I have generated a testing discount for anti-AICDA antibody (ab5197) for use in ChIP:

    Discount code:

    Expiration date:



    This code will ...

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    Customer called. Indeed customer had used this ab5197 a lot previously. Then as it became unavailable, had to switch to ab59361. However he would like to switch back to ab5197, as the results were somewhat better.

    Thank you for the phone call and your patience.

    The laboratory has now provided the images of the results with the new and old batch. The old batch has been tested by a collaborator and the new batch has been tested in the laboratory here - this...

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    I am a scientist in GSF in Germany. I want to use your company antibodies :AID antibody (ab5197) and UNG antibody (ab13668) in western blot. But my proteins come from chicken B cells. I wonder if I can use your antibodies. Attachment is my protein amino a...

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    Thank you for your enquiry regarding ab5197 and ab13668. Having done a BLAST search (for gallus gallus) for the immunogen sequences used to generate those antibodies I found: 92% homology for AID : Query: 1 NVRWAKGRRETYLC 14 N+RWAKG...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"