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Products:Immunology >> Immunoglobulins >> Class Switching
Overview
Product name
Anti-AICDA antibody
See all AICDA products (8) ...
Description
Rabbit polyclonal to AICDA
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide derived from residues 1 - 100 of Human AID.
.
Positive control
This antibody gave a positive signal in AICDA 293T Cell Transient Overexpression Lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
Immunology >> Immunoglobulins >> Class Switching
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Western blot - AICDA antibody (ab5197)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
Target
Function
RNA-editing deaminase involved in somatic hypermutation, gene conversion, and class-switch recombination. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses.
Tissue specificity
Strongly expressed in lymph nodes and tonsils.
Involvement in disease
Defects in AICDA are the cause of hyper-IgM immunodeficiency syndrome type 2 (HIGM2) [MIM:605258]; also known as hyper-IgM syndrome 2. HIGM2 is an autosomal recessive disorder characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE, resulting in a profound susceptibility to bacterial infections. HIGM2 causes the absence of Ig class switch recombination (CSR), the lack of Ig somatic hypermutations, and lymph node hyperplasia caused by the presence of giant germinal centers.
Sequence similarities
Belongs to the cytidine and deoxycytidylate deaminase family.
Target information above from: UniProt accessionQ9GZX7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Activation induced cytidine deaminase antibody
- Activation induced deaminase antibody
- Activation-Induced Cytidine Deaminase antibody
see all
Database links
- Entrez Gene: 57379 Human
- Entrez Gene: 11628 Mouse
- Omim: 605257 Human
- SwissProt: Q9GZX7 Human
- SwissProt: Q9WVE0 Mouse
- Unigene: 149342 Human
- Unigene: 391503 Mouse
Anti-AICDA antibody images:
Western blot - AICDA antibody (ab5197)
Anti-AICDA antibody (ab5197) at 1 µg/ml + AICDA 293T Cell Transient Overexpression Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
Predicted band size : 24 kDa
Observed band size : 24 kDa
References for Anti-AICDA antibody (ab5197)
This product has been referenced in:
- Duquette MLet al. AID binds to transcription-induced structures in c-MYC that map to regions associated with translocation and hypermutation. Oncogene 24:5791-8 (2005).Read more (PubMed: 15940261) »
See 1 publication for this product
Publishing research using ab5197? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"