AIF Human Profiling ELISA Kit (ab126583)
- Product nameAIF Human Profiling ELISA Kit
Intra-assay Sample n Mean SD CV% Cell lysates 4 3.6%
- Tests1 x 96 well plate
- Sample typeTissue Extracts, Cell Lysate
- Assay typeSandwich
- Sensitivity> 6 µg/ml
- Range6 µg/ml - 250 µg/ml
- Species reactivityReacts with: Human
Does not react withMouse, Rat
- Product overview
Apoptosis-inducing factor is a mitochondrial enzyme with probable oxidoreductase activity which functions to regulate Complex I activity in the mitochondrial membrane. When mitochondria are damaged, as in apoptosis, AIF can migrate to the cytosol and nucleus. AIF in the nucleus signals chromatin condensation and DNA fragmentation. This method of programmed cell death (apoptosis) does not require caspase activation.
In addition to its role in apoptosis defects in AIF1 are the cause of combined oxidative phosphorylation deficiency type 6 (COXPD6). COXPD6 is a mitochondrial disease resulting in a neurodegenerative disorder characterized by psychomotor delay, hypotonia, areflexia, muscle weakness and wasting.
- Tested applicationsELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X AIF Detector Antibody 1 x 1ml 10X Blocking Buffer 1 x 6ml 10X HRP Label 1 x 1ml 20X Buffer 1 x 20ml AIF Microplate (12 x 8 antibody coated well strips) 1 unit Extraction Buffer 1 x 15ml TMB Development Solution 1 x 12ml
modificationsUnder normal conditions, a 54-residue N-terminal segment is first proteolytically removed during or just after translocation into the mitochondrial intermembrane space (IMS) by the mitochondrial processing peptidase (MPP) to form the inner-membrane-anchored mature form (AIFmit). During apoptosis, it is further proteolytically processed at amino-acid position 101 leading to the generation of the mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis in a caspase-independent manner.
- Apoptosis-inducing factorApoptosis-inducing factor 1Apoptosis-inducing factor 1, mitochondrialApoptosis-inducing factor, mitochondrion-associated, 1COXPD6mitochondrialPDCD8Programmed cell death 8Programmed cell death protein 8
Our Abpromise guarantee covers the use of ab126583 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
AIF Human Profiling ELISA Kit images
Example serially titrated control sample of HeLa cell extract in the working range of the assay.
Anti-AIF antibody [7F7AB10] (ab110327) at 5 µg/ml +
Human Heart Mitochondrial lysate (ab110337) at 5 µg
Immunocytochemistry analysis of normal untreated HeLa cells with the capture antibody in this kit, ab110327, at 5µg/ml shows staining of AIF is located exclusively to mitochondria. Nuclei were stained with DAPI.
References for AIF Human Profiling ELISA Kit (ab126583)
ab126583 has not yet been referenced specifically in any publications.