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MitoSciences (MS1059)

AIF Human Profiling ELISA Kit (ab126583)

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Overview

  • Product nameAIF Human Profiling ELISA Kit
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Cell lysates 4 3.6%
  • Tests
    1 x 96 well plate
  • Sample type
    Tissue Extracts, Cell Lysate
  • Assay typeSandwich
  • Sensitivity
    > 6 µg/ml
  • Range
    6 µg/ml - 250 µg/ml
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Product overview

    Apoptosis-inducing factor is a mitochondrial enzyme with probable oxidoreductase activity which functions to regulate Complex I activity in the mitochondrial membrane. When mitochondria are damaged, as in apoptosis, AIF can migrate to the cytosol and nucleus. AIF in the nucleus signals chromatin condensation and DNA fragmentation. This method of programmed cell death (apoptosis) does not require caspase activation.

    In addition to its role in apoptosis defects in AIF1 are the cause of combined oxidative phosphorylation deficiency type 6 (COXPD6). COXPD6 is a mitochondrial disease resulting in a neurodegenerative disorder characterized by psychomotor delay, hypotonia, areflexia, muscle weakness and wasting.

  • Tested applicationsELISA more details

Properties

  • FunctionProbable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.
  • Involvement in diseaseDefects in AIFM1 are the cause of combined oxidative phosphorylation deficiency type 6 (COXPD6) [MIM:300816]. It is a mitochondrial disease resulting in a neurodegenerative disorder characterized by psychomotor delay, hypotonia, areflexia, muscle weakness and wasting.
  • Sequence similaritiesBelongs to the FAD-dependent oxidoreductase family.
  • Post-translational
    modifications    Under normal conditions, a 54-residue N-terminal segment is first proteolytically removed during or just after translocation into the mitochondrial intermembrane space (IMS) by the mitochondrial processing peptidase (MPP) to form the inner-membrane-anchored mature form (AIFmit). During apoptosis, it is further proteolytically processed at amino-acid position 101 leading to the generation of the mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis in a caspase-independent manner.
  • Cellular localizationMitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm > perinuclear region. Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region.

    • Target information above from: UniProt accession O95831 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        AIFM1AIFM1_HUMANAIFsh2
        Apoptosis-inducing factorApoptosis-inducing factor 1Apoptosis-inducing factor 1, mitochondrialApoptosis-inducing factor, mitochondrion-associated, 1COXPD6mitochondrialPDCD8Programmed cell death 8Programmed cell death protein 8
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab126583 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      AIF Human Profiling ELISA Kit images

      • ELISA - AIF Human Profiling ELISA Kit (ab126583)
        ELISA - AIF Human Profiling ELISA Kit (ab126583)
        Example serially titrated control sample of HeLa cell extract in the working range of the assay.
      • Western blot - AIF Human Profiling ELISA Kit (ab126583)
        Western blot - AIF Human Profiling ELISA Kit (ab126583)
        Anti-AIF antibody [7F7AB10] (ab110327) at 5 µg/ml + Human Heart Mitochondrial lysate (ab110337) at 5 µg
      • Immunocytochemistry/ Immunofluorescence - AIF Human Profiling ELISA Kit (ab126583)
        Immunocytochemistry/ Immunofluorescence - AIF Human Profiling ELISA Kit (ab126583)
        Immunocytochemistry analysis of normal untreated HeLa cells with the capture antibody in this kit, ab110327, at 5µg/ml shows staining of AIF is located exclusively to mitochondria. Nuclei were stained with DAPI.

      Protocols

      References for AIF Human Profiling ELISA Kit (ab126583)

      ab126583 has not yet been referenced specifically in any publications.

      Publishing research using ab126583 ? Please let us know so that we can cite the reference in this datasheet

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      Has this product ever been tested with serum samples? Would you have any recommendations when using with serum samples? Finally what would you recommend as a 'normal' control when using human serum as sample?

      Thank you for contacting us. I have had the chance to speak with the development group as requested. AIF is an intracellular protein as such this particular assay (ab126583) has not been tested in serum yet. If there is good reason to believe that th...

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