Anti-AIF antibody [4E7] (ab130240)

Overview

• Product nameAnti-AIF antibody [4E7]See all AIF primary antibodies ...
• Description
  Mouse monoclonal [4E7] to AIF
• Tested applicationsWB, ELISA, IHC-P, Flow Cyt, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Human, African Green Monkey
  
• Immunogen

  Purified recombinant fragment of Human AIF expressed in E. Coli.

• Positive controlRecombinant AIF protein; NIH 3T3, Jurkat, HeLa, HepG2, MOLT4, C6, Raji, COS7 and PC12 cell lysate.

Properties

• FormLiquid
• Storage instructionsStore at 4°C or at -20°C for long term storage.
• Storage bufferPreservative: 0.03% Sodium azide
  Constituent: 99% Ascites
• Concentration information loading...
• PurityAscites
• Clonality Monoclonal
• Clone number4E7
• IsotypeIgG2b
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Oxidative stress

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Apoptosis

  • Cancer
  • Invasion/microenvironment
  • Apoptosis
  • Chromatin condensation

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Apoptosis
  • Nuclear

  • Cell Biology
  • Apoptosis
  • Nucleus
  • Chromatin Condensation

Applications

Target

• FunctionProbable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.
• Involvement in diseaseDefects in AIFM1 are the cause of combined oxidative phosphorylation deficiency type 6 (COXPD6) [MIM:300816]. It is a mitochondrial disease resulting in a neurodegenerative disorder characterized by psychomotor delay, hypotonia, areflexia, muscle weakness and wasting.
• Sequence similaritiesBelongs to the FAD-dependent oxidoreductase family.
• Post-translational
  modificationsUnder normal conditions, a 54-residue N-terminal segment is first proteolytically removed during or just after translocation into the mitochondrial intermembrane space (IMS) by the mitochondrial processing peptidase (MPP) to form the inner-membrane-anchored mature form (AIFmit). During apoptosis, it is further proteolytically processed at amino-acid position 101 leading to the generation of the mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis in a caspase-independent manner.
• Cellular localizationMitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm > perinuclear region. Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region.

Target information above from: UniProt accession O95831 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 51060 Human
  • Entrez Gene: 9131 Human
  • Entrez Gene: 9131 Human
  • Entrez Gene: 9131 Human
  • Entrez Gene: 26926 Mouse
  • Entrez Gene: 83533 Rat
  • Omim: 300169 Human
  • SwissProt: O95831 Human

  • SwissProt: O95831 Human
  • SwissProt: O95831 Human
  • SwissProt: Q9Z0X1 Mouse
  • SwissProt: Q9JM53 Rat
  • Unigene: 424932 Human
  • Unigene: 476033 Human
  • Unigene: 240434 Mouse
  • Unigene: 203165 Rat

  see all

• Alternative names
  AIFM1 antibodyAIFM1_HUMAN antibodyApoptosis inducing factor 1, mitochondrial antibody

  Apoptosis inducing factor antibodyApoptosis inducing factor antibodyApoptosis inducing factor, mitochondrion associated, 1 antibodyApoptosis-inducing factor 1 antibodyCOXPD6 antibodyHarlequin antibodyHarlequin antibodyHq antibodyHq antibodymAIF antibodymAIF antibodyMGC111425 antibodyMGC5706 antibodyMGC5706 antibodymitochondrial antibodyPDCD 8 antibodyPDCD8 antibodyPDCD8 antibodyProgrammed cell death 8 (apoptosis inducing factor) antibodyProgrammed cell death 8 antibodyProgrammed cell death 8 isoform 1 antibodyProgrammed cell death 8 isoform 1 antibodyProgrammed cell death 8 isoform 2 antibodyProgrammed cell death 8 isoform 2 antibodyProgrammed cell death 8 isoform 3 antibodyProgrammed cell death 8 isoform 3 antibodyProgrammed cell death protein 8 antibodyProgrammed cell death protein 8 mitochondrial antibodyProgrammed cell death protein 8 mitochondrial precursor antibodyProgrammed cell death protein 8 mitochondrial precursor antibodyStriatal apoptosis inducing factor antibody

  see all

Anti-AIF antibody [4E7] images

• 

  Western blot - Anti-AIF antibody [4E7] (ab130240)
  Anti-AIF antibody [4E7] (ab130240) at 1/500 dilution + recombinant AIF protein.
  
  Predicted band size : 67 kDa
  
• 

  Western blot - Anti-AIF antibody [4E7] (ab130240)
  All lanes : Anti-AIF antibody [4E7] (ab130240) at 1/500 dilution
  
  Lane 1 : NIH 3T3 cell lysate
  Lane 2 : Jurkat cell lysate
  Lane 3 : HeLa cell lysate
  Lane 4 : HepG2 cell lysate
  Lane 5 : MOLT4 cell lysate
  Lane 6 : C6 cell lysate
  Lane 7 : Raji cell lysate
  Lane 8 : Cos7 cell lysate
  Lane 9 : PC12 cell lysate
  
  
  Predicted band size : 67 kDa