Anti-AIF antibody (ab1998)
- Product nameAnti-AIF antibodySee all AIF primary antibodies ...
- DescriptionRabbit polyclonal to AIF
- Tested applicationsWB, IHC-P, ICC/IF more details
- Species reactivityReacts with: Mouse, Rat, Human
Peptide corresponding to amino acids 517 to531 of human AIF. This sequence is identical tothose of mouse and rat AIF.
- Positive control
- K562 cell lysate
- General notesApoptosis Inducing Factor
- Storage instructionsStore at +4°C.
- Storage bufferPBS with 0.02% sodium azide
- Concentration information loading...
- PurityIgG fraction
- Clonality Polyclonal
Our Abpromise guarantee covers the use of ab1998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).Can be blocked with AIF peptide (ab7870). Can be blocked with AIF (internal) peptide (human).|
|IHC-P||IHC-P: Use at an assay dependent dilution.|
|ICC/IF||ICC/IF: Use a concentration of 1 µg/ml.|
- FunctionProbable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.
- Involvement in diseaseDefects in AIFM1 are the cause of combined oxidative phosphorylation deficiency type 6 (COXPD6) [MIM:300816]. It is a mitochondrial disease resulting in a neurodegenerative disorder characterized by psychomotor delay, hypotonia, areflexia, muscle weakness and wasting.
- Sequence similaritiesBelongs to the FAD-dependent oxidoreductase family.
modificationsUnder normal conditions, a 54-residue N-terminal segment is first proteolytically removed during or just after translocation into the mitochondrial intermembrane space (IMS) by the mitochondrial processing peptidase (MPP) to form the inner-membrane-anchored mature form (AIFmit). During apoptosis, it is further proteolytically processed at amino-acid position 101 leading to the generation of the mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis in a caspase-independent manner.
- Cellular localizationMitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm > perinuclear region. Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region.
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Anti-AIF antibody images
All lanes : Anti-AIF antibody (ab1998) at 1 µg/ml
Lane 1 : K562 cell lysate
Lane 2 : Rat heart tissue lysate
Lane 3 : Mouse heart tissue lysate
Predicted band size : 67 kDa
Immunohistochemistry of AIF in human retina with anti-AIF (IN) at 10 µg/ml.
ab1998 staining AIF in human U2OS cells by Immunocytochemistry/ Immunofluorescence.
Samples were fixed using 4% paraformaldehyde.
Left image shows fixed cells labeled with ab1998 (red) and cyclophilin A (green).
Right image shows U2OS cell 6 hours after induction of apoptosis by 200 nM staurosporine.Note translocated of AIF to the nucleus upon induction of apoptosis.
ICC/IF image of ab1998 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1998, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-AIF antibody (ab1998)
This product has been referenced in:
- Ozaki T et al. Inhibitory peptide of mitochondrial µ-calpain protects against photoreceptor degeneration in rhodopsin transgenic S334ter and P23H rats. PLoS One 8:e71650 (2013). IHC ; Mouse . Read more (PubMed: 23951212) »
- Arnandis T et al. Calpains mediate epithelial-cell death during mammary gland involution: mitochondria and lysosomal destabilization. Cell Death Differ : (2012). Read more (PubMed: 22555453) »