Anti-AIF antibody (ab1998)

Overview

• Product nameAnti-AIF antibodySee all AIF primary antibodies ...
• Description
  Rabbit polyclonal to AIF
• Tested applicationsWB, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Peptide corresponding to amino acids 517 to531 of human AIF. This sequence is identical tothose of mouse and rat AIF.

• Positive controlK562 cell lysate
• General notesApoptosis Inducing Factor

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPBS with 0.02% sodium azide
• Concentration information loading...
• PurityIgG fraction
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Oxidative stress

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Apoptosis

  • Cancer
  • Invasion/microenvironment
  • Apoptosis
  • Chromatin condensation

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Apoptosis
  • Nuclear

  • Cell Biology
  • Apoptosis
  • Nucleus
  • Chromatin Condensation

Applications

Target

• FunctionProbable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.
• Involvement in diseaseDefects in AIFM1 are the cause of combined oxidative phosphorylation deficiency type 6 (COXPD6) [MIM:300816]. It is a mitochondrial disease resulting in a neurodegenerative disorder characterized by psychomotor delay, hypotonia, areflexia, muscle weakness and wasting.
• Sequence similaritiesBelongs to the FAD-dependent oxidoreductase family.
• Post-translational
  modificationsUnder normal conditions, a 54-residue N-terminal segment is first proteolytically removed during or just after translocation into the mitochondrial intermembrane space (IMS) by the mitochondrial processing peptidase (MPP) to form the inner-membrane-anchored mature form (AIFmit). During apoptosis, it is further proteolytically processed at amino-acid position 101 leading to the generation of the mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis in a caspase-independent manner.
• Cellular localizationMitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm > perinuclear region. Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region.

Target information above from: UniProt accession O95831 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 51060 Human
  • Entrez Gene: 9131 Human
  • Entrez Gene: 9131 Human
  • Entrez Gene: 9131 Human
  • Entrez Gene: 26926 Mouse
  • Entrez Gene: 83533 Rat
  • Omim: 300169 Human
  • SwissProt: O95831 Human

  • SwissProt: O95831 Human
  • SwissProt: O95831 Human
  • SwissProt: Q9Z0X1 Mouse
  • SwissProt: Q9JM53 Rat
  • Unigene: 424932 Human
  • Unigene: 476033 Human
  • Unigene: 240434 Mouse
  • Unigene: 203165 Rat

  see all

• Alternative names
  AIFM1 antibodyAIFM1_HUMAN antibodyApoptosis inducing factor 1, mitochondrial antibody

  Apoptosis inducing factor antibodyApoptosis inducing factor, mitochondrion associated, 1 antibodyApoptosis-inducing factor 1 antibodyCOXPD6 antibodyHarlequin antibodyHq antibodymAIF antibodyMGC111425 antibodyMGC5706 antibodymitochondrial antibodyPDCD 8 antibodyPDCD8 antibodyProgrammed cell death 8 (apoptosis inducing factor) antibodyProgrammed cell death 8 antibodyProgrammed cell death 8 isoform 1 antibodyProgrammed cell death 8 isoform 2 antibodyProgrammed cell death 8 isoform 3 antibodyProgrammed cell death protein 8 antibodyProgrammed cell death protein 8 mitochondrial antibodyProgrammed cell death protein 8 mitochondrial precursor antibodyStriatal apoptosis inducing factor antibody

  see all

Anti-AIF antibody images

• 

  Western blot - AIF antibody (ab1998)
  All lanes : Anti-AIF antibody (ab1998) at 1 µg/ml
  
  Lane 1 : K562 cell lysate
  Lane 2 : Rat heart tissue lysate
  Lane 3 : Mouse heart tissue lysate
  
  
  Predicted band size : 67 kDa
  
• 

  Immunohistochemistry (Paraffin-embedded sections) - AIF antibody (ab1998)
  Immunohistochemistry of AIF in human retina with anti-AIF (IN) at 10 µg/ml.
• 

  Immunocytochemistry/ Immunofluorescence - AIF antibody (ab1998)Image from Varecha M et al, J Biomed Sci. 2009 Jul 6;16:59, fig 2.
  ab1998 staining AIF in human U2OS cells by Immunocytochemistry/ Immunofluorescence.
  Samples were fixed using 4% paraformaldehyde.
  
  Left image shows fixed cells labeled with ab1998 (red) and cyclophilin A (green).
  Right image shows U2OS cell 6 hours after induction of apoptosis by 200 nM staurosporine.
  Note translocated of AIF to the nucleus upon induction of apoptosis.
• 

  Immunocytochemistry/ Immunofluorescence - AIF antibody (ab1998)
  ICC/IF image of ab1998 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1998, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.