Overview

  • Product nameAnti-AIF antibody
    See all AIF primary antibodies
  • Description
    Rabbit polyclonal to AIF
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    KDGEQHEDLNEVAK

    , corresponding to amino acids 593 - 606 of Human AIF.

  • Positive control
    • K562 whole cell lysate.
  • General notesApoptosis Inducing Factor.

Properties

Applications

Our Abpromise guarantee covers the use of ab2086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
    This antibody can be used with studies using ab1998.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionProbable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.
    • Involvement in diseaseDefects in AIFM1 are the cause of combined oxidative phosphorylation deficiency type 6 (COXPD6) [MIM:300816]. It is a mitochondrial disease resulting in a neurodegenerative disorder characterized by psychomotor delay, hypotonia, areflexia, muscle weakness and wasting.
    • Sequence similaritiesBelongs to the FAD-dependent oxidoreductase family.
    • Post-translational
      modifications
      Under normal conditions, a 54-residue N-terminal segment is first proteolytically removed during or just after translocation into the mitochondrial intermembrane space (IMS) by the mitochondrial processing peptidase (MPP) to form the inner-membrane-anchored mature form (AIFmit). During apoptosis, it is further proteolytically processed at amino-acid position 101 leading to the generation of the mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis in a caspase-independent manner.
    • Cellular localizationMitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm > perinuclear region. Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region.
    • Information by UniProt
    • Database links
    • Alternative names
      • AIFM1 antibody
      • AIFM1_HUMAN antibody
      • Apoptosis inducing factor 1, mitochondrial antibody
      • Apoptosis inducing factor antibody
      • Apoptosis inducing factor, mitochondrion associated, 1 antibody
      • Apoptosis-inducing factor 1 antibody
      • CMTX4 antibody
      • COXPD6 antibody
      • Harlequin antibody
      • Hq antibody
      • mAIF antibody
      • MGC111425 antibody
      • MGC5706 antibody
      • mitochondrial antibody
      • Neuropathy, axonal motor-sensory, with deafness and mental retardation antibody
      • neuropathy, axonal, motor-sensory with deafness and mental retardation (Cowchock syndrome) antibody
      • PDCD 8 antibody
      • PDCD8 antibody
      • Programmed cell death 8 (apoptosis inducing factor) antibody
      • Programmed cell death 8 antibody
      • Programmed cell death 8 isoform 1 antibody
      • Programmed cell death 8 isoform 2 antibody
      • Programmed cell death 8 isoform 3 antibody
      • Programmed cell death protein 8 antibody
      • Programmed cell death protein 8 mitochondrial antibody
      • Programmed cell death protein 8 mitochondrial precursor antibody
      • Striatal apoptosis inducing factor antibody
      see all

    Anti-AIF antibody images

    • All lanes : Anti-AIF antibody (ab2086) at 1 µg/ml

      Lane 1 : K562 cell lysate
      Lane 2 : Mouse liver tissue lysate
      Lane 3 : Rat liver tissue lysate


      Predicted band size : 67 kDa
      Observed band size : 67 kDa

    References for Anti-AIF antibody (ab2086)

    ab2086 has not yet been referenced specifically in any publications.

    Product Wall

    We havn't tested the chicken reactivity for any one. However, the immunogenic peptide sequences of the 2267 (IN) and the 2301 (CT) are highly conserved (identical) in human, mouse and rat. They are likely to be broad species response. If you would like...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"