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Product name
AIF peptide
See all AIF products (4) ...
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.1% BSA 0.02% sodium azide pH7.2
Concentration
Concentration information loading...
Sequence notes
KQKKAALSASEGEE Amino acids 109 to 122 of human AIF (1).
Research areas
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Metabolism >> Pathways and Processes >> Redox metabolism >> Oxidative stress
Cancer >> Invasion/microenvironment >> Apoptosis >> Chromatin condensation
Epigenetics and Nuclear Signaling >> Cell cycle >> Apoptosis >> Nuclear
Cell Biology >> Apoptosis >> Nucleus >> Chromatin Condensation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7871 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking
Application notes
BL: Use at an assay dependent concentration.
This peptide can be used with studies using ab1999.
It usually blocks the antibody activity completely in Western blot by incubating the peptide with an equal volume of antibody for 30 min at 37°C
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Function
Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.
Involvement in disease
Defects in AIFM1 are the cause of combined oxidative phosphorylation deficiency type 6 (COXPD6) [MIM:300816]. It is a mitochondrial disease resulting in a neurodegenerative disorder characterized by psychomotor delay, hypotonia, areflexia, muscle weakness and wasting.
Sequence similarities
Belongs to the FAD-dependent oxidoreductase family.
Post-translational
modifications
Under normal conditions, a 54-residue N-terminal segment is first proteolytically removed during or just after translocation into the mitochondrial intermembrane space (IMS) by the mitochondrial processing peptidase (MPP) to form the inner-membrane-anchored mature form (AIFmit). During apoptosis, it is further proteolytically processed at amino-acid position 101 leading to the generation of the mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis in a caspase-independent manner.
Cellular localization
Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm > perinuclear region. Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region.
Target information above from: UniProt accessionO95831
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
References for AIF peptide (ab7871)
ab7871 has not yet been referenced specifically in any publications.
Publishing research using ab7871? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"