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Western blot - AIFM3 antibody (ab83791)
Western blot - AIFM3 antibody (ab83791)
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Product Name 

AIFM3 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to AIFM3

Immunogen 

Synthetic peptide (EGFSDRIVLC TLDRHLPYDR PKLSKSLDTQ PEQLALRPKE FFRAYGIEVL) corresponding to a region within internal sequence amino acids 216-265 of human AIFM3 (NP_653305).
BLAST 'EGFSDRIVLC TLDRHLPYDR PKLSKSLDTQ PEQLALRPKE FFRAYGIEVL ' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution. Titre using peptide based assay: 1/312500.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 67 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HeLa cell lysate

Cellular localization 

Endoplasmic reticulum. Mitochondrial inner membrane.

Research areas 

Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins

Relevance 

AIFM3, a resident mitochondrial protein, is involved in the induction of aopotosis through a caspase-dependent process. It is also responsible for reducing mitochondrial membrane potential. There are three different isoforms.

 

AIFM3 antibody (ab83791)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

150209    (Human)

SwissProt

    

Q96NN9    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Purification notes 

Purified by peptide affinity chromatography method.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this AIFM3 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for AIFM3 antibody images, references, products related to ab83791 and other tools.

 

AIFM3 antibody images:

Product image  Western blot - AIFM3 antibody (ab83791)

Western blot - AIFM3 antibody (ab83791)

AIFM3 antibody (ab83791) at 1 µg/ml + HeLa cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 67 kDa
Observed band size : 64 kDa (why is the actual band size different from the predicted?)

 
 

Search PubMed (MEDLINE) for references to AIFM3

 

AIFM3 antibody - more information

Customer reviews (feedback) regarding AIFM3 antibody

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AIFM3 antibody - related products:

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AIFM3 antibody - other tools:

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