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Anti-AIFM3 antibody (ab83791)

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Overview

Product name

Anti-AIFM3 antibody
See all AIFM3 products (3) ...

Description

Rabbit polyclonal to AIFM3

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Chicken, Cow

Immunogen

Synthetic peptide (EGFSDRIVLC TLDRHLPYDR PKLSKSLDTQ PEQLALRPKE FFRAYGIEVL) corresponding to a region within internal sequence amino acids 216-265 of human AIFM3 (NP_653305).

EGFSDRIVLC TLDRHLPYDR PKLSKSLDTQ PEQLALRPKE FFRAYGIEVL

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

HeLa cell lysate

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Purified by peptide affinity chromatography method.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins

  • Western blot - AIFM3 antibody (ab83791)Western blot - AIFM3 antibody (ab83791) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab83791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: Use at an assay dependent dilution. Titre using peptide based assay: 1/312500.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 67 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Induces apoptosis through a caspase dependent pathway. Reduces mitochondrial membrane potential.

Tissue specificity

Ubiquitous. Expressed in bone marrow, cerebral cortex, liver, ovary, thymus, thyroid gland and tongue (at protein level).

Sequence similarities

Belongs to the FAD-dependent oxidoreductase family.
Contains 1 Rieske domain.

Domain

The Rieske domain induces apoptosis.

Cellular localization

Mitochondrion. Does not translocate to the nucleus upon induction of apoptosis.

Target information above from: UniProt accessionQ96NN9 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • AIFL antibody
  • Aifm3 antibody
  • AIFM3_HUMAN antibody
  • Apoptosis inducing factor 3 antibody
  • Apoptosis inducing factor like antibody
  • Apoptosis inducing factor like protein antibody
  • Apoptosis inducing factor, mitochondrion associated antibody
  • Apoptosis-inducing factor 3 antibody
  • Apoptosis-inducing factor-like protein antibody
  • FLJ30473 antibody
  • FLJ45137 antibody
see all

Database links

Anti-AIFM3 antibody images:

  Western blot - AIFM3 antibody (ab83791)

Western blot - AIFM3 antibody (ab83791)

Anti-AIFM3 antibody (ab83791) at 1 µg/ml + HeLa cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 67 kDa
Observed band size : 64 kDa (why is the actual band size different from the predicted?)

References for Anti-AIFM3 antibody (ab83791)

ab83791 has not yet been referenced specifically in any publications.

Publishing research using ab83791? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"