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Product Name
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AKAP12 antibody [JP74]
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See all AKAP12 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [JP74] to AKAP12
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Immunogen
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Tissue / cell preparation (Human) - fractionated membrane cortex proteins from neuron cell lines NT2-N and SK-N-BE(2).
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Reacts with
(species key)
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Hu, Ms
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Specificity
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This antibody recognizes Gravin (AKAP12) (approx. 300 kDa).
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Tested applications
(see key)
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ICC, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 0.02 - 0.04 µg/ml. Detects a band of approximately 300 kDa (predicted molecular weight: 191 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Rat brain cytosolic S1 fraction.
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Cellular localization
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Cytoplasm; cell cortex (Probable). Cytoplasm; cytoskeleton (Probable). May be part of the cortical cytoskeleton.
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Research areas
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Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Signal Transduction >> Adapters >> Cytoplasmic
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Relevance
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Gravin (also known as A Kinase Anchor Protein 12, AKAP12) was cloned from a cDNA expression library screened with serum, from a myasthenia gravis patient. Antibodies to Gravin were found in 22 out of 72 myasthemia gravis patients and absent in sera of normal individuals. This protein is a myristoylated high molecular weight cytoplasmic protein expressed in endothelial cells, cultured fibroblasts and osteosarcoma cells and highly expressed in the brain. Gravin can bind kinases like PKA and PKC by different domains of the protein and can localize these kinases to specific domains in the cell. Upon differentiation of NT2 cells to neurons (NT2 N), Gravin expression is up regulated and enriched at the inner peripheral cortex in close proximity to the plasma membrane. In differentiated cells, gravin is co-localized with PKCbII, PKCa and PKA. Decreased binding of PKCa to gravin was found after activation of PKC.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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JP74
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Myeloma
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x63-Ag8.653
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Isotype
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IgG2a
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Purity
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IgG fraction
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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2.500 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this AKAP12 antibody [JP74] is on this datasheet. But please do contact us if you would like any reassurance! |
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References for AKAP12 antibody [JP74] (ab49849)
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This product has been used in: (two most recent references)
Schnizler K et al. Protein kinase A anchoring via AKAP150 is essential for TRPV1 modulation by forskolin and prostaglandin E2 in mouse sensory neurons. J Neurosci 28:4904-17 (2008). WB; Mouse. PubMed: 18463244
This clone has been used in:
If you publish research using ab49849 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to AKAP12
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