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Products:Signal Transduction >> Adapters >> Cytoplasmic
Overview
Product name
Anti-AKAP12 antibody [JP74]
See all AKAP12 products (4) ...
Description
Mouse monoclonal [JP74] to AKAP12
Specificity
This antibody recognizes Gravin (AKAP12) (approx. 300 kDa).
Tested applications
WB, IP, ICCmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Tissue / cell preparation (Human) - fractionated membrane cortex proteins from neuron cell lines NT2-N and SK-N-BE(2).
Positive control
Rat brain cytosolic S1 fraction.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
JP74
Myeloma
x63-Ag8.653
Isotype
IgG2a
Research areas
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Signal Transduction >> Adapters >> Cytoplasmic
Applications
Show applications key
Our Abpromise guarantee covers the use of ab49849 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.02 - 0.04 µg/ml.Detects a band of approximately 300 kDa (predicted molecular weight: 191 kDa).
IP: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
Target
Function
Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).
Tissue specificity
Expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells.
Sequence similarities
Contains 3 AKAP domains.
Domain
Polybasic regions located between residues 266 and 557 are involved in binding PKC.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. May be part of the cortical cytoskeleton.
Target information above from: UniProt accessionQ02952
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A kinase (PRKA) anchor protein (gravin) 12 antibody
- A kinase Anchor Protein 12 antibody
- A kinase Anchor Protein 12 antibody
see all
Database links
- Entrez Gene: 9590 Human
- Entrez Gene: 83397 Mouse
- Omim: 604698 Human
- SwissProt: Q02952 Human
- SwissProt: Q9WTQ5 Mouse
- Unigene: 197081 Human
- Unigene: 371240 Human
- Unigene: 27481 Mouse
References for Anti-AKAP12 antibody [JP74] (ab49849)
This product has been referenced in:
- Gao Set al. AKAP12 and AKAP5 form higher-order hetero-oligomers. J Mol Signal 6:8 (2011). WB; Human.Read more (PubMed: 21831305) »
- Schnizler Ket al. Protein kinase A anchoring via AKAP150 is essential for TRPV1 modulation by forskolin and prostaglandin E2 in mouse sensory neurons. J Neurosci 28:4904-17 (2008). WB; Mouse.Read more (PubMed: 18463244) »
See all 3 publications for this product
Publishing research using ab49849? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"