AKAP12 antibody (ab10346)

Product Name 

AKAP12 antibody

Product type 

Primary antibodies

Description 

Sheep polyclonal to AKAP12

Immunogen 

Full length native protein (purified) (Rat).

Reacts with 
(species key)

Rat

Tested applications 
(see key)

IP, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
IP: Use at a concentration of 2 - 5 µg/ml.
WB: Use at a concentration of 2 - 5 µg/ml. Predicted molecular weight: 172 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasm; cell cortex (Probable). Cytoplasm; cytoskeleton (Probable). May be part of the cortical cytoskeleton.

Research areas 

Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Signal Transduction >> Adapters >> Cytoplasmic

Relevance 

Gravin (also known as A Kinase Anchor Protein 12, AKAP12) was cloned from a cDNA expression library screened with serum, from a myasthenia gravis patient. Antibodies to Gravin were found in 22 out of 72 myasthemia gravis patients and absent in sera of normal individuals. This protein is a myristoylated high molecular weight cytoplasmic protein expressed in endothelial cells, cultured fibroblasts and osteosarcoma cells and highly expressed in the brain. Gravin can bind kinases like PKA and PKC by different domains of the protein and can localize these kinases to specific domains in the cell. Upon differentiation of NT2 cells to neurons (NT2 N), Gravin expression is up regulated and enriched at the inner peripheral cortex in close proximity to the plasma membrane. In differentiated cells, gravin is co-localized with PKCbII, PKCa and PKA. Decreased binding of PKCa to gravin was found after activation of PKC.

AKAP12 is a major protein kinase C substrate with tumor suppressor activity in models of src- and ras-induced oncogenic transformation. AKAP12 regulates actin-based cytoskeletal and tissue architecture, as well as kinases such as protein kinase C (PKC), protein kinase A (PKA) and calmodulin. Additionally, AKAP12 has been implicated in the formation of migratory processes and cell migration during embryogenesis. Rat AKAP12 shares extensive homology with human Gravin, an autoantigen in myasthenia gravis that encodes kinase scaffolding functions and whose expression pattern in fibroblasts and nerves suggests a role in cell motility.

Database links 

Raised in 

Sheep

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: 0.08% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Working dilution samples should be discarded if not used within 12 hours.

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