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Anti-AKAP12 antibody (ab10346)

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Overview

Product name

Anti-AKAP12 antibody
See all AKAP12 products (4) ...

Description

Sheep polyclonal to AKAP12

Tested applications

WB, IPmore details

Cross reactivity

Reacts with

Rat

Immunogen

Full length native protein (purified) (Rat).

General notes

Working dilution samples should be discarded if not used within 12 hours.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.08% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Protein A purified

Primary antibody notes

AKAP12 is a major protein kinase C substrate with tumor suppressor activity in models of src- and ras-induced oncogenic transformation. AKAP12 regulates actin-based cytoskeletal and tissue architecture, as well as kinases such as protein kinase C (PKC), protein kinase A (PKA) and calmodulin. Additionally, AKAP12 has been implicated in the formation of migratory processes and cell migration during embryogenesis. Rat AKAP12 shares extensive homology with human Gravin, an autoantigen in myasthenia gravis that encodes kinase scaffolding functions and whose expression pattern in fibroblasts and nerves suggests a role in cell motility.

Clonality

Polyclonal

Isotype

IgG

Applications

Show applications key

Our Abpromise guarantee covers the use of ab10346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 2 - 5 µg/ml.Predicted molecular weight: 172 kDa.

  • ShowHide

    IP

     IP: Use a concentration ...Read more →

    IP: Use a concentration of 2 - 5 µg/ml.

Target

Function

Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).

Tissue specificity

Expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells.

Sequence similarities

Contains 3 AKAP domains.

Domain

Polybasic regions located between residues 266 and 557 are involved in binding PKC.

Post-translational
modifications

Phosphorylated upon DNA damage, probably by ATM or ATR.

Cellular localization

Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. May be part of the cortical cytoskeleton.

Target information above from: UniProt accessionQ02952 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • A kinase (PRKA) anchor protein (gravin) 12 antibody
  • A kinase Anchor Protein 12 antibody
  • A kinase anchor protein 250kDa antibody
  • A-kinase anchor protein 12 antibody
  • A-kinase anchor protein 250 kDa antibody
  • AKA12_HUMAN antibody
  • AKAP 12 antibody
  • AKAP 250 antibody
  • AKAP-12 antibody
  • Akap12 antibody
  • AKAP250 antibody
  • DKFZp686M0430 antibody
  • DKFZp686O0331 antibody
  • FLJ20945 antibody
  • FLJ97621 antibody
  • Gercelin antibody
  • Gravin antibody
  • Kinase scaffold protein gravin antibody
  • Myasthenia gravis autoantigen antibody
  • Myasthenia gravis autoantigen gravin antibody
  • OTTHUMP00000017426 antibody
  • OTTHUMP00000017427 antibody
  • Src-Suppressed C Kinase Substrate antibody
  • Srcs5 antibody
  • SSeCKS antibody
  • Tsga12 antibody
see all

References for Anti-AKAP12 antibody (ab10346)

This product has been referenced in:

  • Zan Let al. Temporal profile of Src, SSeCKS, and angiogenic factors after focal cerebral ischemia: correlations with angiogenesis and cerebral edema. Neurochem Int 58:872-9 (2011). WB, IHC-P; Rat.Read more (PubMed: 21334414) »

See 1 publication for this product

Publishing research using ab10346? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"