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Products:Signal Transduction >> Adapters >> Cytoplasmic
Overview
Product name
Anti-AKAP12 antibody
See all AKAP12 products (4) ...
Description
Sheep polyclonal to AKAP12
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Rat
Immunogen
Full length native protein (purified) (Rat).
General notes
Working dilution samples should be discarded if not used within 12 hours.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.08% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Primary antibody notes
AKAP12 is a major protein kinase C substrate with tumor suppressor activity in models of src- and ras-induced oncogenic transformation. AKAP12 regulates actin-based cytoskeletal and tissue architecture, as well as kinases such as protein kinase C (PKC), protein kinase A (PKA) and calmodulin. Additionally, AKAP12 has been implicated in the formation of migratory processes and cell migration during embryogenesis. Rat AKAP12 shares extensive homology with human Gravin, an autoantigen in myasthenia gravis that encodes kinase scaffolding functions and whose expression pattern in fibroblasts and nerves suggests a role in cell motility.
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Signal Transduction >> Adapters >> Cytoplasmic
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 2 - 5 µg/ml.Predicted molecular weight: 172 kDa.
IP: Use a concentration of 2 - 5 µg/ml.
Target
Function
Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).
Tissue specificity
Expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells.
Sequence similarities
Contains 3 AKAP domains.
Domain
Polybasic regions located between residues 266 and 557 are involved in binding PKC.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. May be part of the cortical cytoskeleton.
Target information above from: UniProt accessionQ02952
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A kinase (PRKA) anchor protein (gravin) 12 antibody
- A kinase Anchor Protein 12 antibody
- A kinase anchor protein 250kDa antibody
see all
Database links
- Entrez Gene: 83425 Rat
- SwissProt: Q5QD51 Rat
- Unigene: 122094 Rat
- Unigene: 42891 Rat
References for Anti-AKAP12 antibody (ab10346)
This product has been referenced in:
- Zan Let al. Temporal profile of Src, SSeCKS, and angiogenic factors after focal cerebral ischemia: correlations with angiogenesis and cerebral edema. Neurochem Int 58:872-9 (2011). WB, IHC-P; Rat.Read more (PubMed: 21334414) »
See 1 publication for this product
Publishing research using ab10346? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"