Feedback Form

Product Name 

AKAP12 antibody

 

See all AKAP12 antibodies (5)...

Product type 

Primary antibodies

Description 

Sheep polyclonal to AKAP12

Immunogen 

Full length native protein (purified) (Rat).

Reacts with 
(species key)

Rat

Tested applications 
(see key)

IP, WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews

Submit an Abreview

Be the first to review this antibody and earn 220 Abpoints.


Application notes 
(see key)

Recommended dilutions
IP: Use at a concentration of 2 - 5 µg/ml.
WB: Use at a concentration of 2 - 5 µg/ml. Predicted molecular weight: 172 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasm; cell cortex (Probable). Cytoplasm; cytoskeleton (Probable). May be part of the cortical cytoskeleton.

Research areas 

Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Signal Transduction >> Adapters >> Cytoplasmic

Relevance 

Gravin (also known as A Kinase Anchor Protein 12, AKAP12) was cloned from a cDNA expression library screened with serum, from a myasthenia gravis patient. Antibodies to Gravin were found in 22 out of 72 myasthemia gravis patients and absent in sera of normal individuals. This protein is a myristoylated high molecular weight cytoplasmic protein expressed in endothelial cells, cultured fibroblasts and osteosarcoma cells and highly expressed in the brain. Gravin can bind kinases like PKA and PKC by different domains of the protein and can localize these kinases to specific domains in the cell. Upon differentiation of NT2 cells to neurons (NT2 N), Gravin expression is up regulated and enriched at the inner peripheral cortex in close proximity to the plasma membrane. In differentiated cells, gravin is co-localized with PKCbII, PKCa and PKA. Decreased binding of PKCa to gravin was found after activation of PKC.

AKAP12 is a major protein kinase C substrate with tumor suppressor activity in models of src- and ras-induced oncogenic transformation. AKAP12 regulates actin-based cytoskeletal and tissue architecture, as well as kinases such as protein kinase C (PKC), protein kinase A (PKA) and calmodulin. Additionally, AKAP12 has been implicated in the formation of migratory processes and cell migration during embryogenesis. Rat AKAP12 shares extensive homology with human Gravin, an autoantigen in myasthenia gravis that encodes kinase scaffolding functions and whose expression pattern in fibroblasts and nerves suggests a role in cell motility.

 

Alternative names AKAP12 antibody (ab10346)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

83425    (Rat)

Unigene

    

42891    (Rat)

Raised in 

Sheep

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: 0.08% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Working dilution samples should be discarded if not used within 12 hours.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this AKAP12 antibody is on this datasheet. But please do contact us if you would like any reassurance!


 

Search PubMed (MEDLINE) for references to AKAP12

 

AKAP12 antibody - more information

Customer reviews (feedback) regarding AKAP12 antibody

Customer FAQs regarding AKAP12 antibody

Protocols for AKAP12 antibody

Price and availability of products related to AKAP12 antibody

 

AKAP12 antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Compatible Secondaries for AKAP12 antibody

ab6743

  

FITC antibody

ab6745

  

Texas Red ® antibody

ab6746

  

Biotin antibody

ab6747

  

HRP antibody

ab6748

  

Alkaline Phosphatase antibody

ab7009

  

Phycoerythrin antibody

 

Other AKAP12 Antibodies

See all AKAP12 antibodies (5)...

 

AKAP12 antibody - other tools:

Contact Abcam with a Technical Enquiry about AKAP12 antibody

 

All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

©1998-2010 Abcam plc. All rights reserved.