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Western blot - AKAP12 antibody (ab9698)
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Product Name
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AKAP12 antibody
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See all AKAP12 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to AKAP12
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Immunogen
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Synthetic peptide: C-KQERESAKSELTES, corresponding to C terminal amino acids 1769-1782 of Human AKAP12(Peptide available as ab23159.)
BLAST 'C-KQERESAKSELTES' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 268 kDa (predicted molecular weight: 196 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Jurkat and HeLa cell lysates
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Cellular localization
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Cytoplasm; cell cortex (Probable). Cytoplasm; cytoskeleton (Probable). May be part of the cortical cytoskeleton.
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Research areas
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Chromatin and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Signal Transduction >> Adapters >> Cytoplasmic
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKA
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Phosphatases
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Relevance
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Gravin (also known as A Kinase Anchor Protein 12, AKAP12) was cloned from a cDNA expression library screened with serum, from a myasthenia gravis patient. Antibodies to Gravin were found in 22 out of 72 myasthemia gravis patients and absent in sera of normal individuals. This protein is a myristoylated high molecular weight cytoplasmic protein expressed in endothelial cells, cultured fibroblasts and osteosarcoma cells and highly expressed in the brain. Gravin can bind kinases like PKA and PKC by different domains of the protein and can localize these kinases to specific domains in the cell. Upon differentiation of NT2 cells to neurons (NT2 N), Gravin expression is up regulated and enriched at the inner peripheral cortex in close proximity to the plasma membrane. In differentiated cells, gravin is co-localized with PKCbII, PKCa and PKA. Decreased binding of PKCa to gravin was found after activation of PKC.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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Tris saline, 0.02% Na azide, pH7.3 with 0.5% BSA Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this AKAP12 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for AKAP12 antibody images, references, products related to ab9698 and other tools.
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AKAP12 antibody images:
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 Western blot - AKAP12 antibody (ab9698)
All lanes : AKAP12 antibody (ab9698) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)
Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate (ab3957)
Lysates/proteins at 20 µg per lane.
Secondary
Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 196 kDa
Observed band size : 268 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa,30 kDa,45 kDa. We are unsure as to the identity of these extra bands.
AKAP12 contains a number of potential phosphorylation sites (SwissProt data) which may explain it running at a higher molecular weight than predicted. The 268 kDa band is comparable to molecular weights seen with other commercially available antibodies to AKAP12.
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References for AKAP12 antibody (ab9698)
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This product has been used in:
Lynch MJ et al. RNA silencing identifies PDE4D5 as the functionally relevant cAMP phosphodiesterase interacting with beta arrestin to control the protein kinase A/AKAP79-mediated switching of the beta2-adrenergic receptor to activation of ERK in HEK293B2 cells. J Biol Chem 280:33178-89 (2005). PubMed: 16030021
If you publish research using ab9698 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to AKAP12
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AKAP12 antibody - more information
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