For the best experience on the Abcam website please upgrade to a modern browser such as
Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer Tris saline, 0.02% Na azide, pH7.3 with 0.5% BSA
Concentration information loading...
Purity IgG fraction
Purification notes Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 268 kDa (predicted molecular weight: 196 kDa).Can be blocked with
Human AKAP12 peptide (ab23159).
Function Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).
Tissue specificity Expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells.
Sequence similarities Contains 3 AKAP domains.
Domain Polybasic regions located between residues 266 and 557 are involved in binding PKC.
Post-translational modifications Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. May be part of the cortical cytoskeleton.
Information by UniProt
A kinase (PRKA) anchor protein (gravin) 12 antibody
A kinase Anchor Protein 12 antibody
A kinase anchor protein 250kDa antibody
Anti-AKAP12 antibody images
Western blot - AKAP12 antibody (ab9698)
All lanes : Anti-AKAP12 antibody (ab9698) at 1 µg/ml Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate ( ab27252) Lane 2 : SW480 (Human) Whole Cell Lysate ( ab3957) Lysates/proteins at 20 µg per lane. Secondary Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) ( ab65486) at 1/3000 dilution Performed under reducing conditions. Predicted band size : 196 kDa Observed band size : 268 kDa ( why is the actual band size different from the predicted?) Additional bands at : 100 kDa,30 kDa,45 kDa. We are unsure as to the identity of these extra bands.
References for Anti-AKAP12 antibody (ab9698)
This product has been referenced in:
et al. RNA silencing identifies PDE4D5 as the functionally relevant cAMP phosphodiesterase interacting with beta arrestin to control the protein kinase A/AKAP79-mediated switching of the beta2-adrenergic receptor to activation of ERK in HEK293B2 cells. J Biol Chem 280:33178-89 (2005).
Read more (PubMed: 16030021) »
See 1 Publication
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"