Anti-AKAP12 antibody (ab9698)

Overview

• Product nameAnti-AKAP12 antibodySee all AKAP12 primary antibodies ...
• Description
  Goat polyclonal to AKAP12
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide: C-KQERESAKSELTES, corresponding to C terminal amino acids 1769-1782 of Human AKAP12(Peptide available as ab23159.)

  Run BLAST with Run BLAST with
• Positive controlJurkat and HeLa cell lysates

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferTris saline, 0.02% Na azide, pH7.3 with 0.5% BSA
• Concentration information loading...
• PurityIgG fraction
• Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Adapters
  • Cytoplasmic

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • PKA

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • PKC

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Phosphatases

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Kinases/Phosphatases
  • Other

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Other

Applications

Target

• FunctionAnchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).
• Tissue specificityExpressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells.
• Sequence similaritiesContains 3 AKAP domains.
• DomainPolybasic regions located between residues 266 and 557 are involved in binding PKC.
• Post-translational
  modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
• Cellular localizationCytoplasm > cell cortex. Cytoplasm > cytoskeleton. May be part of the cortical cytoskeleton.

Target information above from: UniProt accession Q02952 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 9590 Human
  • Entrez Gene: 9590 Human
  • Omim: 604698 Human
  • SwissProt: Q02952 Human
  • SwissProt: Q02952 Human
  • Unigene: 197081 Human
  • Unigene: 371240 Human

• Alternative names
  A kinase (PRKA) anchor protein (gravin) 12 antibodyA kinase Anchor Protein 12 antibodyA kinase Anchor Protein 12 antibody

  A kinase anchor protein 250kDa antibodyA-kinase anchor protein 12 antibodyA-kinase anchor protein 250 kDa antibodyAKA12_HUMAN antibodyAKAP 12 antibodyAKAP 250 antibodyAKAP-12 antibodyAKAP12 antibodyAKAP250 antibodyDKFZp686M0430 antibodyDKFZp686O0331 antibodyFLJ20945 antibodyFLJ97621 antibodyGercelin antibodyGravin antibodyKinase scaffold protein gravin antibodyMyasthenia gravis autoantigen antibodyMyasthenia gravis autoantigen gravin antibodyOTTHUMP00000017426 antibodyOTTHUMP00000017427 antibodySrc-Suppressed C Kinase Substrate antibodySrcs5 antibodySSeCKS antibodyTsga12 antibody

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Anti-AKAP12 antibody images

• 

  Western blot - AKAP12 antibody (ab9698)
  All lanes : Anti-AKAP12 antibody (ab9698) at 1 µg/ml
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)
  Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate (ab3957)
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 196 kDa
  Observed band size : 268 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 100 kDa,30 kDa,45 kDa. We are unsure as to the identity of these extra bands.