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Western blot - AKAP12 antibody (ab54473)
(enlarge)
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Product Name
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AKAP12 antibody [mAbcam54473]
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See all AKAP12 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [mAbcam54473] to AKAP12
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 750 - 850 of Human AKAP12. (Peptide available as ab58432.)
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Reacts with
(species key)
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Hu, Rat
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 10 µg/ml. Detects a band of approximately 260,191 kDa (predicted molecular weight: 191 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following lysates:
Hela Whole Cell
SW480 Whole Cell
Rat Colon Tissue
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Cellular localization
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Cytoplasm; cell cortex (Probable). Cytoplasm; cytoskeleton (Probable). May be part of the cortical cytoskeleton.
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Research areas
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Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Signal Transduction >> Adapters >> Cytoplasmic
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Relevance
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Gravin (also known as A Kinase Anchor Protein 12, AKAP12) was cloned from a cDNA expression library screened with serum, from a myasthenia gravis patient. Antibodies to Gravin were found in 22 out of 72 myasthemia gravis patients and absent in sera of normal individuals. This protein is a myristoylated high molecular weight cytoplasmic protein expressed in endothelial cells, cultured fibroblasts and osteosarcoma cells and highly expressed in the brain. Gravin can bind kinases like PKA and PKC by different domains of the protein and can localize these kinases to specific domains in the cell. Upon differentiation of NT2 cells to neurons (NT2 N), Gravin expression is up regulated and enriched at the inner peripheral cortex in close proximity to the plasma membrane. In differentiated cells, gravin is co-localized with PKCbII, PKCa and PKA. Decreased binding of PKCa to gravin was found after activation of PKC.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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mAbcam54473
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Myeloma
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Sp2/0-Ag14
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS, pH7.4
Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.30 - 0.80 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this AKAP12 antibody [mAbcam54473] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for AKAP12 antibody [mAbcam54473] images, references, products related to ab54473 and other tools.
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AKAP12 antibody [mAbcam54473] images:
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 Western blot - AKAP12 antibody (ab54473)
All lanes : AKAP12 antibody [mAbcam54473] (ab54473) at 10 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : Colon (Rat) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 191 kDa
Observed band size : 191,260 kDa (why is the actual band size different from the predicted?)
Additional bands at : 41 kDa,50 kDa,90 kDa. We are unsure as to the identity of these extra bands.
AKAP12 contains a number of potential phosphorylation sites (SwissProt data) which may explain it running at a higher molecular weight than predicted. The 260 kDa band is comparable to molecular weights seen with other commercially available antibodies to AKAP12
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Search PubMed (MEDLINE) for references to AKAP12
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AKAP12 antibody [mAbcam54473] - more information
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Customer reviews (feedback) regarding AKAP12 antibody [mAbcam54473] |
Customer FAQs regarding AKAP12 antibody [mAbcam54473] |
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Price and availability of products related to AKAP12 antibody [mAbcam54473] |
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AKAP12 antibody [mAbcam54473] - related products:
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AKAP12 antibody [mAbcam54473] - other tools:
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Contact Abcam with a Technical Enquiry about AKAP12 antibody [mAbcam54473] |
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