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Anti-AKAP13 antibody (ab56916) at 5 µg/ml + 0.2 µg/lane of partial recombinant AKAP13 protein with a tag
Secondary
Goat anti mouse IgG (H&L)-HRP conjugate at 1/5000 dilution
Predicted band size : 308 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Note: The difference between predicted and actual band sizes may be due to the fact that ab56916 was tested against a partial recombinant AKAP13 protein, and not the full length protein.
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