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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKA
Overview
Product name
Anti-AKAP13 antibody
See all AKAP13 products (6) ...
Description
Mouse monoclonal to AKAP13
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment, corresponding to amino acids 1-111 of Human AKAP13
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Cardiovascular >> Heart >> Hypertrophy >> Other
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Signal Transduction >> Adapters >> Cytoplasmic
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKA
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Western blot - AKAP13 antibody (ab56916)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab56916 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 1-5 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Anchors cAMP-dependent protein kinase (PKA) and acts as an adapter protein to selectively couple G alpha-13 and Rho. Augments gene activation by the estrogen receptor in an element-specific and ligand-dependent manner. Activates estrogen receptor beta by a p38 MAPK-dependent pathway. Isoform 6 stimulates exchange activity on Rho proteins in vitro, but not on CDC42, Ras or Rac and may bind calcium ions.
Tissue specificity
Isoform 3 and isoform 6 are found in hematopoietic cells, skeletal muscle, lung, heart, estrogen-responsive reproductive tissues, including breast ductal epithelium. Also found in testis and breast cancer cell lines. Isoform 6 is not found in brain, placenta, liver, pancreas or kidney. Isoform 7 is expressed in myeloid and lymphoid lineages, a variety of epithelial tissues and skeletal muscle. Isoform 2 is predominantly found in the heart and at lower levels in the lung, placenta, kidney, pancreas, skeletal muscle and liver.
Sequence similarities
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Domain
Both the DH and PH domains are required for transforming activity.
Cellular localization
Cytoplasm; Cytoplasm. Nucleus and Membrane.
Target information above from: UniProt accessionQ12802
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A kinase (PRKA) anchor protein 13 antibody
- A kinase anchor protein 13 antibody
- A kinase anchoring protein antibody
see all
Database links
- Entrez Gene: 11214 Human
- Omim: 604686 Human
- SwissProt: Q12802 Human
- Unigene: 459211 Human
- Unigene: 710656 Human
Anti-AKAP13 antibody images:
Western blot - AKAP13 antibody (ab56916)
Anti-AKAP13 antibody (ab56916) at 5 µg/ml + 0.2 µg/lane of partial recombinant AKAP13 protein with a tag
Secondary
Goat anti mouse IgG (H&L)-HRP conjugate at 1/5000 dilution
Predicted band size : 308 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Note: The difference between predicted and actual band sizes may be due to the fact that ab56916 was tested against a partial recombinant AKAP13 protein, and not the full length protein.
References for Anti-AKAP13 antibody (ab56916)
ab56916 has not yet been referenced specifically in any publications.
Publishing research using ab56916? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"