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Western blot - AKAP13 antibody (ab56916)
(enlarge)
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Product Name
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AKAP13 antibody
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See all AKAP13 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to AKAP13
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Immunogen
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Recombinant fragment, corresponding to amino acids 1-111 of Human AKAP13
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Reacts with
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Hu
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Tested applications
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1-5 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Isoform 2: Cytoplasm.
Isoform 3: Cytoplasm. Nucleus.
Isoform 6: Cytoplasm.
Isoform 7: Membrane.
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Research areas
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Cardiovascular >> Heart >> Hypertrophy >> Other
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Signal Transduction >> Adapters >> Cytoplasmic
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKA
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Relevance
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AKAP13 functions as a protein kinase A (PKA)-anchoring protein and acts as an adapter protein to selectively couple G alpha-13 and Rho. It contains a dbl oncogene homology (DH) domain and a pleckstrin homology (PH) domain. The DH domain is associated with guanine nucleotide exchange activation for the Rho/Rac family of small GTP binding proteins. AKAP13 augments gene activation by the estrogen receptor and activates estrogen receptor beta by a p38 MAPK-dependent pathway.
AKAP13 is involved in the signaling pathways leading to cardiomyocytes hypertrophy.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG2a
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
PBS, pH 7.2
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this AKAP13 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for AKAP13 antibody images, references, products related to ab56916 and other tools.
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AKAP13 antibody images:
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 Western blot - AKAP13 antibody (ab56916)
AKAP13 antibody (ab56916) at 5 µg/ml + 0.2 µg/lane of partial recombinant AKAP13 protein with a tag
Secondary
Goat anti mouse IgG (H&L)-HRP conjugate at 1/5000 dilution
Predicted band size : 308 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Note: The difference between predicted and actual band sizes may be due to the fact that ab56916 was tested against a partial recombinant AKAP13 protein, and not the full length protein.
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Search PubMed (MEDLINE) for references to AKAP13
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AKAP13 antibody - more information
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Customer reviews (feedback) regarding AKAP13 antibody |
Customer FAQs regarding AKAP13 antibody |
Protocols for AKAP13 antibody |
Price and availability of products related to AKAP13 antibody |
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AKAP13 antibody - related products:
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AKAP13 antibody - other tools:
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Contact Abcam with a Technical Enquiry about AKAP13 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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