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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKA
Overview
Product name
Anti-AKAP13 antibody
See all AKAP13 products (6) ...
Description
Rabbit polyclonal to AKAP13
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide derived from internal sequence of human AKAP13.
Positive control
Human lung carcinoma tissue. Extracts from Jurkat and A549 cells.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Hypertrophy >> Other
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Signal Transduction >> Adapters >> Cytoplasmic
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKA
Applications
Show applications key
Our Abpromise guarantee covers the use of ab74048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Predicted molecular weight: 308 kDa.
ELISA
ELISA: 1/20000ELISA: 1/20000
IHC-P: 1/50 - 1/100.
Target
Function
Anchors cAMP-dependent protein kinase (PKA) and acts as an adapter protein to selectively couple G alpha-13 and Rho. Augments gene activation by the estrogen receptor in an element-specific and ligand-dependent manner. Activates estrogen receptor beta by a p38 MAPK-dependent pathway. Isoform 6 stimulates exchange activity on Rho proteins in vitro, but not on CDC42, Ras or Rac and may bind calcium ions.
Tissue specificity
Isoform 3 and isoform 6 are found in hematopoietic cells, skeletal muscle, lung, heart, estrogen-responsive reproductive tissues, including breast ductal epithelium. Also found in testis and breast cancer cell lines. Isoform 6 is not found in brain, placenta, liver, pancreas or kidney. Isoform 7 is expressed in myeloid and lymphoid lineages, a variety of epithelial tissues and skeletal muscle. Isoform 2 is predominantly found in the heart and at lower levels in the lung, placenta, kidney, pancreas, skeletal muscle and liver.
Sequence similarities
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Domain
Both the DH and PH domains are required for transforming activity.
Cellular localization
Cytoplasm; Cytoplasm. Nucleus and Membrane.
Target information above from: UniProt accessionQ12802
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A kinase (PRKA) anchor protein 13 antibody
- A kinase anchor protein 13 antibody
- A kinase anchoring protein antibody
see all
Database links
- Entrez Gene: 11214 Human
- Omim: 604686 Human
- SwissProt: Q12802 Human
- Unigene: 459211 Human
- Unigene: 710656 Human
Anti-AKAP13 antibody images:
Western blot - AKAP13 antibody (ab74048)
All lanes : Anti-AKAP13 antibody (ab74048) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from A549 cells
Lane 3 : Extracts from Jurkat cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 308 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AKAP13 antibody (ab74048)
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using ab74048, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
References for Anti-AKAP13 antibody (ab74048)
ab74048 has not yet been referenced specifically in any publications.
Publishing research using ab74048? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"