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Western blot - AKAP13 antibody (ab74048)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AKAP13 antibody (ab74048)
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Product Name
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AKAP13 antibody
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See all AKAP13 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to AKAP13
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Immunogen
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Synthetic peptide derived from internal sequence of human AKAP13.
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/20000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Predicted molecular weight: 308 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human lung carcinoma tissue. Extracts from Jurkat and A549 cells.
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Cellular localization
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Isoform 2: Cytoplasm.
Isoform 3: Cytoplasm. Nucleus.
Isoform 6: Cytoplasm.
Isoform 7: Membrane.
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Research areas
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Cardiovascular >> Heart >> Hypertrophy >> Other
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Signal Transduction >> Adapters >> Cytoplasmic
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKA
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Relevance
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AKAP13 functions as a protein kinase A (PKA)-anchoring protein and acts as an adapter protein to selectively couple G alpha-13 and Rho. It contains a dbl oncogene homology (DH) domain and a pleckstrin homology (PH) domain. The DH domain is associated with guanine nucleotide exchange activation for the Rho/Rac family of small GTP binding proteins. AKAP13 augments gene activation by the estrogen receptor and activates estrogen receptor beta by a p38 MAPK-dependent pathway.
AKAP13 is involved in the signaling pathways leading to cardiomyocytes hypertrophy.
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AKAP13 antibody (ab74048) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this AKAP13 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for AKAP13 antibody images, references, products related to ab74048 and other tools.
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AKAP13 antibody images:
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 Western blot - AKAP13 antibody (ab74048)
All lanes : AKAP13 antibody (ab74048) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from A549 cells
Lane 3 : Extracts from Jurkat cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 308 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AKAP13 antibody (ab74048)
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using ab74048, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
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Search PubMed (MEDLINE) for references to AKAP13
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AKAP13 antibody - more information
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Customer reviews (feedback) regarding AKAP13 antibody |
Customer FAQs regarding AKAP13 antibody |
Protocols for AKAP13 antibody |
Price and availability of products related to AKAP13 antibody |
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AKAP13 antibody - related products:
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AKAP13 antibody - other tools:
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Contact Abcam with a Technical Enquiry about AKAP13 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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