Recombinant Anti-AKAP14 antibody [EPR12955] - C-terminal (ab180160)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12955] to AKAP14 - C-terminal
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-AKAP14 antibody [EPR12955] - C-terminal
See all AKAP14 primary antibodies -
Description
Rabbit monoclonal [EPR12955] to AKAP14 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Jurkat, HUVEC, NCI-H460, A549 and Human fetal kidney lysates; A549 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12955 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180160 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
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IP |
1/10 - 1/100.
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ICC/IF |
1/100 - 1/250.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 23 kDa. |
IP
1/10 - 1/100. |
ICC/IF
1/100 - 1/250. |
Target
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Relevance
Binds to type II regulatory subunits of protein kinase A and anchors/targets them -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 158798 Human
- Entrez Gene: 434756 Mouse
- Entrez Gene: 60332 Rat
- Omim: 300462 Human
- SwissProt: Q86UN6 Human
- SwissProt: O35817 Rat
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Alternative names
- A kinase (PRKA) anchor protein 14 antibody
- A kinase anchor protein 14 antibody
- A kinase anchor protein 28 kDa antibody
see all
Images
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All lanes : Anti-AKAP14 antibody [EPR12955] - C-terminal (ab180160) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HUVEC cell lysate
Lane 3 : NCI-H460 cell lysate
Lane 4 : A549 cell lysate
Lane 5 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 23 kDa -
Immunofluorescence analysis of A549 cells labeling AKAP14 with ab180160 at a 1/100 dilution.
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Western blot analysis on Immunoprecipitation pellet from either 1) Jurkat cell lysate, or 2) 1xPBS (negative control); showing AKAP14, using ab180160 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab180160 has not yet been referenced specifically in any publications.