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Product name
Anti-AKAP8L antibody
See all AKAP8L products (2) ...
Description
Rabbit polyclonal to AKAP8L
Tested applications
ELISA, WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Dog
Immunogen
A region within synthetic peptide: GAVSPPPPPP PEEEEEGAVP LLGGALQRQI RGIPGLDVED DEEGGGGAP, corresponding to C terminal amino acids 598-646 of Human AKAP8L
GAVSPPPPPPPEEEEEGAVPLLGGALQRQIRGIPGLDVED DEEGGGGAP
Positive control
Jurkat cell lysate, human lung and human kidney tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab51342 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ELISA: Use at an assay dependent dilution. Peptide ELISA: 1/312500.
IHC-P: Use at a concentration of 4 - 8 µg/ml.
WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
AKAP8L could play a role in constitutive transport element (CTE)-mediated gene expression. It does not seem to be implicated in the binding of regulatory subunit II of PKA. It may be involved in nuclear envelope breakdown and chromatin condensation. It may regulate the initiation phase of DNA replication when associated with TMPO-beta.
Cellular localization
Nucleus; nuclear matrix. Cytoplasm. Note=Nuclear at steady state but shuttles between the nucleus and cytoplasm. Associated with the nuclear matrix.
Alternative names
- A kinase (PRKA) anchor protein 8 like antibody
- AKAP 8L antibody
- A kinase anchor protein 8 like antibody
see all
Database links
- Entrez Gene: 26993 Human
- Entrez Gene: 54194 Mouse
- Entrez Gene: 299569 Rat
- Omim: 609475 Human
- SwissProt: Q9ULX6 Human
- SwissProt: Q9R0L7 Mouse
- SwissProt: Q3TLG2 Mouse
Anti-AKAP8L antibody images:
Western blot - AKAP8L antibody (ab51342)
Anti-AKAP8L antibody (ab51342) at 1.25 µg/ml + Jurkat cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG (1:100,000)
Predicted band size : 72 kDa
Observed band size : 72 kDa
Immunohistochemistry (Paraffin-embedded sections) - AKAP8L antibody (ab51342)
Ab51342 (8ug/ml) staining human AKAP8L in human lung tissue by immunohistochemistry using paraffin embedded tissue. Alveolar cells (indicated with arrows) were positively stained.
Immunohistochemistry (Paraffin-embedded sections) - AKAP8L antibody (ab51342)
Ab51342 (8ug/ml) staining human AKAP8L in human kidney tissue by immunohistochemistry using paraffin embedded tissue. Epithelial cells of renal tubule (indicated with arrows) were positively stained.
References for Anti-AKAP8L antibody (ab51342)
This product has been referenced in:
- Cohen AA et al. Dynamic proteomics of individual cancer cells in response to a drug. Science 322:1511-6 (2008). WB; Human.Read more (PubMed: 19023046) »
See 1 publication for this product
Publishing research using ab51342? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"