AKIP 293T Transfected Lysate - (positive control) (ab94297)

Overview

  • Product nameAKIP 293T Transfected Lysate - (positive control)
  • General notesab94297 is a 293T cell transfected lysate in which Human AKIP has been transiently over-expressed using a pCMV-AKIP plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsWBmore details

Properties

  • BackgroundFunction: May act as a negative regulator of Aurora-A kinase, by down-regulation through proteasome-dependent degradation. Tissue specificity: Ubiquitously expressed and especially highly expressed in heart, skeletal muscle and testis.

Applications

Our Abpromise guarantee covers the use of ab94297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

AKIP 293T Transfected Lysate - (positive control) images

  • ab94297 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-AKIP antibody (ab67343) at 1/500 dilution

    Lane 1 : AKIP 293T Transfected Lysate - (positive control) (ab94297)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for AKIP 293T Transfected Lysate - (positive control) (ab94297)

ab94297 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"