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Products:Signal Transduction >> Metabolism >> Energy Metabolism
Overview
Product name
Anti-AKR1B10 antibody
See all AKR1B10 products (7) ...
Description
Rabbit polyclonal to AKR1B10
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Fruit fly (Drosophila melanogaster), Zebrafish
Immunogen
Synthetic peptide corresponding to a sequence within N terminal amino acids 37-86 of Human AKR1B10. (DAGYRHIDCA YVYQNEHEVG EAIQEKIQEK AVKREDLFIV SKLWPTFFER)
DAGYRHIDCA YVYQNEHEVG EAIQEKIQEK AVKREDLFIV SKLWPTFFER
Positive control
A549 cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Enzymes >> Other
Signal Transduction >> Metabolism >> Drug metabolism
Signal Transduction >> Metabolism >> Energy Metabolism
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Western blot - AKR1B10 antibody (ab62218)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab62218 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
- 1 Image
WB
Application notes
ELISA: Use at an assay dependent dilution. Titre using peptide based assay: 1:1562500
WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Acts as all-trans-retinaldehyde reductase. Can efficiently reduce aliphatic and aromatic aldehydes, and is less active on hexoses (in vitro). May be responsible for detoxification of reactive aldehydes in the digested food before the nutrients are passed on to other organs.
Tissue specificity
Found in many tissues. Highly expressed in small intestine, colon and adrenal gland.
Sequence similarities
Belongs to the aldo/keto reductase family.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionO60218
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Aldose reductase like antibody
- Aldose reductase related protein antibody
- ARL 1 antibody
see all
Database links
- Entrez Gene: 57016 Human
- Entrez Gene: 67861 Mouse
- Entrez Gene: 296972 Rat
- Omim: 604707 Human
- SwissProt: O60218 Human
- Unigene: 116724 Human
- Unigene: 8475 Rat
Anti-AKR1B10 antibody images:
Western blot - AKR1B10 antibody (ab62218)
Anti-AKR1B10 antibody (ab62218) at 1.25 µg/ml + A549 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 35 kDa
Observed band size : 35 kDa
References for Anti-AKR1B10 antibody (ab62218)
ab62218 has not yet been referenced specifically in any publications.
Publishing research using ab62218? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"