Anti-AKR1C1 antibody [4B6AF3] (ab131375)

Overview

• Product nameAnti-AKR1C1 antibody [4B6AF3]See all AKR1C1 primary antibodies ...
• Description
  Mouse monoclonal [4B6AF3] to AKR1C1
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  Predicted to work with: Rabbit, Cow
• Immunogen

  Purified protein cocktail.

• Positive controlWB: HeLa, HepG2, HLH, HHH, MLH, RLH and RHH tissue samples ICC/IF: Hela cells

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPreservative: 0.02% Sodium azide
  Constituents: 0.357% HEPES, 0.877% Sodium chloride
• Concentration information loading...
• Purity>90% by SDS-PAGE
• Clonality Monoclonal
• Clone number4B6AF3
• IsotypeIgG2b
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Drug metabolism

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Developmental Biology
  • Reproduction
  • Hormones

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

  • Signal Transduction
  • Metabolism
  • Drug metabolism

  • Signal Transduction
  • Growth Factors/Hormones
  • Hormones

Applications

Target

• FunctionConverts progesterone to its inactive form, 20-alpha-dihydroxyprogesterone (20-alpha-OHP). In the liver and intestine, may have a role in the transport of bile. May have a role in monitoring the intrahepatic bile acid concentration. Has a low bile-binding ability. May play a role in myelin formation.
• Tissue specificityExpressed in all tissues tested including liver, prostate, testis, adrenal gland, brain, uterus, mammary gland and keratinocytes. Highest levels found in liver, mammary gland and brain.
• Sequence similaritiesBelongs to the aldo/keto reductase family.
• Cellular localizationCytoplasm.

Target information above from: UniProt accession Q04828 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1645 Human
  • Entrez Gene: 77337 Mouse
  • Entrez Gene: 307092 Rat
  • Omim: 600449 Human
  • SwissProt: Q04828 Human
  • SwissProt: Q91WR5 Mouse
  • SwissProt: Q6AYQ2 Rat
  • Unigene: 460260 Human

  • Unigene: 102679 Rat

  see all

• Alternative names
  2-dihydrobenzene-1 antibody2-diol dehydrogenase antibody20 alpha (3 alpha) hydroxysteroid dehydrogenase antibody

  20 ALPHA HSD antibody20 alpha hydroxysteroid dehydrogenase antibody20-alpha-HSD antibody20-alpha-hydroxysteroid dehydrogenase antibody20ALPHAHSD antibody2ALPHAHSD antibodyAK1C1 antibodyAK1C1_HUMAN antibodyAKR1C1 antibodyAldo keto reductase family 1 member C1 antibodyAldo-keto reductase family 1 member C1 antibodyC9 antibodyChlordecone reductase homolog antibodyChlordecone reductase homolog HAKRC antibodyDD1 antibodyDD1/DD2 antibodyDDH antibodyDDH1 antibodyDihydrodiol dehydrogenase 1 antibodyDihydrodiol dehydrogenase 1/2 antibodydihydrodiol dehydrogenase isoform DD1 antibodyH37 antibodyHAKRC antibodyHBAB antibodyHepatic dihydrodiol dehydrogenase antibodyHigh affinity hepatic bile acid-binding protein antibodyHigh-affinity hepatic bile acid-binding protein antibodyIndanol dehydrogenase antibodyMBAB antibodyMGC8954 antibodyTrans-1 antibodyTrans-1,2 dihydrobenzene 1,2 diol dehydrogenase antibodyType II 3 alpha hydroxysteroid dehydrogenase antibody

  see all

Anti-AKR1C1 antibody [4B6AF3] images

• 

  Western blot - Anti-AKR1C1 antibody [4B6AF3] (ab131375)
  All lanes : Anti-AKR1C1 antibody [4B6AF3] (ab131375) at 2 µg/ml
  
  Lane 1 : Molecular weight marker
  Lane 2 : HeLa Cell Lysate at 15 µg
  Lane 3 : HepG2 Cell Lysate at 15 µg
  Lane 4 : Human Liver Homogenate (HLH) at 10 µg
  Lane 5 : Human Heart Homogenate (HHH) at 10 µg
  Lane 6 : Mouse Liver Homogenate (MLH) at 10 µg
  Lane 7 : Rat Liver Homogenate (RLH) at 10 µg
  Lane 8 : Rat Heart Homogenate (RHH) at 10 µg
  
  Secondary
  Goat anti-Mouse AP at 1/3000 dilution
  
  Predicted band size : 37 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - Anti-AKR1C1 antibody [4B6AF3] (ab131375)
  Immunofluorescence analysis of AKR1C1 in HeLa cells, using ab131375 at 5 µg/ml.
  The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 min). The cells were then incubated with ab131375 for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. 1% BSA was used as the blocking agent for all blocking steps. The target protein locates to the cytosol.