Recombinant Anti-AKR1C1+AKR1C2+AKR1C3 antibody [EPR16725] (ab203834)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16725] to AKR1C3 + AKR1C2 + AKR1C1
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-AKR1C1+AKR1C2+AKR1C3 antibody [EPR16725] -
Description
Rabbit monoclonal [EPR16725] to AKR1C3 + AKR1C2 + AKR1C1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal brain, fetal kidney and fetal liver lysates; MOLT-4 cell lysate. IP: Human fetal liver whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16725 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
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IP |
1/50.
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Notes |
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WB
1/5000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). |
IP
1/50. |
Target
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Cellular localization
AKR1C3: Cytoplasm. AKR1C2: Cytoplasm. AKR1C1: Cytoplasm. -
Database links
- Entrez Gene: 1645 Human
- Entrez Gene: 1646 Human
- Entrez Gene: 8644 Human
- Omim: 600449 Human
- Omim: 600450 Human
- Omim: 603966 Human
- SwissProt: P42330 Human
- SwissProt: P52895 Human
see all
Images
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All lanes : Anti-AKR1C1+AKR1C2+AKR1C3 antibody [EPR16725] (ab203834) at 1/1000 dilution
Lane 1 : His-tagged human AKR1C1 recombinant protein, full-length
Lane 2 : His-tagged human AKR1C2 recombinant protein, full-length
Lane 3 : His-tagged human AKR1C3 recombinant protein, full-length
Lane 4 : His-tagged human AKR1C4 recombinant protein, full-length
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Exposure time: 40 secondsBlocking and diluting buffer: 5% NFDM/TBST
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Anti-AKR1C1+AKR1C2+AKR1C3 antibody [EPR16725] (ab203834) at 1/5000 dilution + Human fetal brain tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Anti-AKR1C1+AKR1C2+AKR1C3 antibody [EPR16725] (ab203834) at 1/10000 dilution + MOLT-4 (Human lymphoblastic leukemia cell line) cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-AKR1C1+AKR1C2+AKR1C3 antibody [EPR16725] (ab203834) at 1/10000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 3 minutesBlocking buffer: 5% NFDM/TBST.
Dilution buffer: 5% NFDM /TBST or 1%BSA /TBST.
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AKR1C3 was immunoprecipitated from 1mg of Human fetal liver whole cell lysate with ab203834 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab203834 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
Lane 1: Human fetal liver whole cell lysate 10ug (Input). Lane 2: ab203834 IP in Human fetal liver whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203834 in Human fetal liver whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab203834 has been referenced in 2 publications.
- Cui X et al. siAKR1C3@PPA complex nucleic acid nanoparticles inhibit castration-resistant prostate cancer in vitro. Front Oncol 12:1069033 (2022). PubMed: 36591491
- Zhou M et al. A Mansonone Derivative Coupled with Monoclonal Antibody 4D5-Modified Chitosan Inhibit AKR1C3 to Treat Castration-Resistant Prostate Cancer. Int J Nanomedicine 15:3087-3098 (2020). PubMed: 32431503