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Anti-AKR1C3 antibody (ab27491) at 0.03 µg/ml + Human breast lysate (35µg protein in RIPA buffer).
developed using the ECL technique
Predicted band size : 37 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour.
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