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Western blot - AKR1C3 antibody (ab27491)
(enlarge)
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Product Name
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AKR1C3 antibody
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See all AKR1C3 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to AKR1C3
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Immunogen
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Synthetic peptide: CFASHPNYPYSDEY, corresponding to C terminal amino acids 311-323 of Human AKR1C3.
BLAST 'CFASHPNYPYSDEY' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/32000.
WB: Use at a concentration of 0.01 - 0.1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human liver and breast lysates for Western blot.
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Cellular localization
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Cytoplasmic
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Research areas
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Neuroscience >> Endocrine system >> Gonadotrophic axis
Signal Transduction >> Metabolism >> Energy Metabolism
Signal Transduction >> Growth Factors/Hormones >> Hormones
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Relevance
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This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20 alpha hydroxy progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9alpha,11beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation.
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AKR1C3 antibody (ab27491) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide.
Constituents: 0.5% BSA, Tris saline. pH 7.3. Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this AKR1C3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for AKR1C3 antibody images, references, products related to ab27491 and other tools.
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AKR1C3 antibody images:
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 Western blot - AKR1C3 antibody (ab27491)
AKR1C3 antibody (ab27491) at 0.03 µg/ml + Human breast lysate (35µg protein in RIPA buffer).
developed using the ECL technique
Predicted band size : 37 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour.
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References for AKR1C3 antibody (ab27491)
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This product has been used in:
Gavelov M et al. Reduction of doxorubicin and oracin and induction of carbonyl reductase in human breast carcinoma MCF-7 cells. Chem Biol Interact 176:9-18 (2008). WB; Human. PubMed: 18755171
If you publish research using ab27491 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to AKR1C3
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AKR1C3 antibody - more information
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Customer reviews (feedback) regarding AKR1C3 antibody |
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AKR1C3 antibody - related products:
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AKR1C3 antibody - other tools:
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