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Anti-AKR1C3 antibody (ab27491)

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Overview

Product name

Anti-AKR1C3 antibody
See all AKR1C3 products (5) ...

Description

Goat polyclonal to AKR1C3

Tested applications

ELISA, WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide: CFASHPNYPYSDEY, corresponding to C terminal amino acids 311-323 of Human AKR1C3.

CFASHPNYPY SDEY

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Human liver and breast lysates for Western blot.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality

Polyclonal

Isotype

IgG

Research areas

Neuroscience >> Endocrine system >> Gonadotrophic axis
Signal Transduction >> Metabolism >> Energy Metabolism
Signal Transduction >> Growth Factors/Hormones >> Hormones

  • Western blot - AKR1C3 antibody (ab27491)Western blot - AKR1C3 antibody (ab27491) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab27491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     ELISA: 1/32000

    ELISA: 1/32000

  • ShowHide1 Image

    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 0.01 - 0.1 µg/ml.Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).

Target

Function

Catalyzes the conversion of aldehydes and ketones to alcohols. Catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ) and the oxidation of 9-alpha,11-beta-PGF2 to PGD2. Functions as a bi-directional 3-alpha-, 17-beta- and 20-alpha HSD. Can interconvert active androgens, estrogens and progestins with their cognate inactive metabolites. Preferentially transforms androstenedione (4-dione) to testosterone.

Tissue specificity

Expressed in many tissues including adrenal gland, brain, kidney, liver, lung, mammary gland, placenta, small intestine, colon, spleen, prostate and testis. The dominant HSD in prostate and mammary gland. In the prostate, higher levels in epithelial cells than in stromal cells. In the brain, expressed in medulla, spinal cord, frontotemporal lobes, thalamus, subthalamic nuclei and amygdala. Weaker expression in the hippocampus, substantia nigra and caudate.

Sequence similarities

Belongs to the aldo/keto reductase family.

Cellular localization

Cytoplasm.

Target information above from: UniProt accessionP42330 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • brain antibody
  • 17 beta HSD 5 antibody
  • 17 beta hydroxysteroid dehydrogenase type 5 antibody
  • 17-beta-HSD 5 antibody
  • 17-beta-hydroxysteroid dehydrogenase type 5 antibody
  • 2-dihydrobenzene-1 antibody
  • 2-diol dehydrogenase antibody
  • 3 alpha hydroxysteroid dehydrogenase type II antibody
  • 3-alpha-HSD type 2 antibody
  • 3-alpha-HSD type II antibody
  • 3-alpha-hydroxysteroid dehydrogenase type 2 antibody
  • AK1C3_HUMAN antibody
  • AKR1 C3 antibody
  • AKR1C3 antibody
  • Aldo keto reductase family 1 member C3 antibody
  • Aldo-keto reductase family 1 member C3 antibody
  • Chlordecone reductase antibody
  • Chlordecone reductase homolog antibody
  • Chlordecone reductase homolog HAKRb antibody
  • DD-3 antibody
  • DD3 antibody
  • DDH1 antibody
  • Dihydrodiol dehydrogenase 3 antibody
  • Dihydrodiol dehydrogenase type I antibody
  • HA1753 antibody
  • HAKRB antibody
  • HAKRe antibody
  • HluPGFS antibody
  • HSD17B5 antibody
  • Hydroxysteroid (17 beta) dehydrogenase 5 antibody
  • Indanol dehydrogenase antibody
  • KIAA0119 antibody
  • PGFS antibody
  • Prostaglandin F synthase antibody
  • Testosterone 17-beta-dehydrogenase 5 antibody
  • Trans-1 antibody
  • Trans12 dihydrobenzene 12 diol dehydrogenase antibody
  • Type IIb 3 alpha hydroxysteroid dehydrogenase antibody
see all

Database links

Anti-AKR1C3 antibody images:

  Western blot - AKR1C3 antibody (ab27491)

Western blot - AKR1C3 antibody (ab27491)

Anti-AKR1C3 antibody (ab27491) at 0.03 µg/ml + Human breast lysate (35µg protein in RIPA buffer).
developed using the ECL technique

Predicted band size : 37 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)


Primary incubation was 1 hour.

References for Anti-AKR1C3 antibody (ab27491)

This product has been referenced in:

  • Phillips RJet al. Genes for prostaglandin synthesis, transport and inactivation are differentially expressed in human uterine tissues, and the prostaglandin F synthase AKR1B1 is induced in myometrial cells by inflammatory cytokines. Mol Hum Reprod 17:1-13 (2011).Read more (PubMed: 20595240) »
  • Bresson Eet al. The Human Aldose Reductase AKR1B1 Qualifies as the Primary Prostaglandin F Synthase in the Endometrium. J Clin Endocrinol Metab : (2010).Read more (PubMed: 20943776) »

See all 3 publications for this product

Publishing research using ab27491? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"