Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Growth Factors/Hormones >> Hormones
Overview
Product name
Anti-AKR1C3 antibody
See all AKR1C3 products (5) ...
Description
Rabbit polyclonal to AKR1C3
Tested applications
WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
A 15 amino acid peptide corresponding to the C terminal residues of human AKR1C3 (P42330).
Positive control
Human fetal liver lysates; human lung tissue; HeLa cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 2% BSA
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Endocrine system >> Gonadotrophic axis
Signal Transduction >> Metabolism >> Energy Metabolism
Signal Transduction >> Growth Factors/Hormones >> Hormones
Applications
Show applications key
Our Abpromise guarantee covers the use of ab84327 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Predicted molecular weight: 36 kDa.
IHC-P: 1/100 - 1/500.
ICC/IF: 1/50 - 1/200.
Target
Function
Catalyzes the conversion of aldehydes and ketones to alcohols. Catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ) and the oxidation of 9-alpha,11-beta-PGF2 to PGD2. Functions as a bi-directional 3-alpha-, 17-beta- and 20-alpha HSD. Can interconvert active androgens, estrogens and progestins with their cognate inactive metabolites. Preferentially transforms androstenedione (4-dione) to testosterone.
Tissue specificity
Expressed in many tissues including adrenal gland, brain, kidney, liver, lung, mammary gland, placenta, small intestine, colon, spleen, prostate and testis. The dominant HSD in prostate and mammary gland. In the prostate, higher levels in epithelial cells than in stromal cells. In the brain, expressed in medulla, spinal cord, frontotemporal lobes, thalamus, subthalamic nuclei and amygdala. Weaker expression in the hippocampus, substantia nigra and caudate.
Sequence similarities
Belongs to the aldo/keto reductase family.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP42330
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- brain antibody
- 17 beta HSD 5 antibody
- 17 beta hydroxysteroid dehydrogenase type 5 antibody
see all
Database links
- Entrez Gene: 8644 Human
- Omim: 603966 Human
- SwissProt: P42330 Human
- Unigene: 78183 Human
Anti-AKR1C3 antibody images:
Western blot - AKR1C3 antibody (ab84327)
Anti-AKR1C3 antibody (ab84327) at 1/500 dilution + human fetal liver lysate
Predicted band size : 36 kDa
Observed band size : 36 kDa
Immunocytochemistry/ Immunofluorescence - AKR1C3 antibody (ab84327)
ab84327, at a 1/50 dilution, staining AKR1C3 in HeLa cells by Immunofluorescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AKR1C3 antibody (ab84327)
ab84327, at a 1/100 dilution, staining AKR1C3 in formalin fixed, paraffin embedded human lung tissue by Immunohistochemistry. Note cytoplasmic staining.
References for Anti-AKR1C3 antibody (ab84327)
ab84327 has not yet been referenced specifically in any publications.
Publishing research using ab84327? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"