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AKR1C3 protein (His tag) (ab87417)

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Overview

Product name

AKR1C3 protein (His tag)
See all AKR1C3 products (2) ...

Protein description

Recombinant full length Human AKR1C3 (amino acids 1-323) fused to a His tag at the N-terminus; MWt 39kDa (343 amino acids) (Swiss-Prot P42330).

Expression host

E. coli

Properties

Purity

>95% by SDS-PAGE

Purification notes

ab87417 is purified using conventional chromatography techniques.

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 10% Glycerol, 20mM Tris HCl, pH 8.0

Concentration

Concentration information loading...

Additional notes

Endotoxin: < 1.0 EU per 1 µg of protein (determined by LAL method).

Sequence notes

MGSSHHHHHH SSGLVPRGSH MDSKHQCVKL NDGHFMPVLG FGTYAPPEVP RSKALEVTKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWSTFH RPELVRPALE NSLKKAQLDY VDLYLIHSPM SLKPGEELSP TDENGKVIFD IVDLCTTWEA MEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNRS KLLDFCKSKD IVLVAYSALG SQRDKRWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTA EDMKAIDGLD RNLHYFNSDS FASHPNYPYS DEY

Research areas

Neuroscience >> Endocrine system >> Gonadotrophic axis
Signal Transduction >> Metabolism >> Energy Metabolism
Signal Transduction >> Growth Factors/Hormones >> Hormones

  • SDS-PAGE - AKR1C3 protein (His tag) (ab87417)SDS-PAGE - AKR1C3 protein (His tag) (ab87417) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab87417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Protein info

Function

Catalyzes the conversion of aldehydes and ketones to alcohols. Catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ) and the oxidation of 9-alpha,11-beta-PGF2 to PGD2. Functions as a bi-directional 3-alpha-, 17-beta- and 20-alpha HSD. Can interconvert active androgens, estrogens and progestins with their cognate inactive metabolites. Preferentially transforms androstenedione (4-dione) to testosterone.

Tissue specificity

Expressed in many tissues including adrenal gland, brain, kidney, liver, lung, mammary gland, placenta, small intestine, colon, spleen, prostate and testis. The dominant HSD in prostate and mammary gland. In the prostate, higher levels in epithelial cells than in stromal cells. In the brain, expressed in medulla, spinal cord, frontotemporal lobes, thalamus, subthalamic nuclei and amygdala. Weaker expression in the hippocampus, substantia nigra and caudate.

Sequence similarities

Belongs to the aldo/keto reductase family.

Cellular localization

Cytoplasm.

Target information above from: UniProt accessionP42330 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

AKR1C3 protein (His tag) images:

  SDS-PAGE - AKR1C3 protein (His tag) (ab87417)

SDS-PAGE - AKR1C3 protein (His tag) (ab87417)

15% SDS-PAGE showing 3µg ab87417 at approximately 39kDa.

References for AKR1C3 protein (His tag) (ab87417)

ab87417 has not yet been referenced specifically in any publications.

Publishing research using ab87417? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"