• Product name
    AKT 1/2/3 pT308 + AKT1 ELISA Kit
  • Detection method
  • Precision
    Sample n Mean SD CV%
    AKT (pT308) 6 1.9%
    AKT1 (Total) 6 4%
    Sample n Mean SD CV%
    AKT (pT308) 3 6.6%
    AKT1 (Total) 3 4.9%
  • Sample type
    Cell Lysate, Tissue Homogenate
  • Assay type
  • Sensitivity
    30 pg/ml
  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Product overview

    Abcam’s AKT 1/2/3 (pT308) and AKT1 (Total) in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the semi-quantitative measurement of AKT 1/2/3 (pT308) and total AKT1 protein in Human and mouse cells.

    The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Estimated sensitivity: Phospho-AKT1/2/3 (Thr308): 0.5 ng/mL (tested with recombinant protein), Total AKT1: 0.2 ng/mL (tested with recombinant protein)
    Range: Phospho-AKT1/2/3 (Thr308): 1-100 ng/mL, Total AKT1: 0.5-50 ng/mL

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Relevance
    AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.
  • Cellular localization
    Nucleus. Cytoplasm. Membrane; Peripheral membrane protein. Note: Membrane-associated after cell stimulation leading to its translocation.
  • Alternative names
    • AKT1
    • AKT2
    • AKT3
    • HIHGHH
    • PKBB
    • PRKBB
    • RAC-BETA
    • v-akt murine thymoma viral oncogene homolog 2
    see all
  • Database links


Our Abpromise guarantee covers the use of ab176658 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

AKT 1/2/3 pT308 + AKT1 ELISA Kit images

  • Example of a typical AKT (pT308) and AKT1 (Total) cell lysate dilution series. Background-subtracted data values (mean +/- SD) are graphed.

  • Linearity of dilution in representative sample matrices. Cellular lysates were prepared at 3 concentrations in common media containing 1X Cell Extraction Buffer PTR. Data from duplicate measurements of AKT1 (pT308) are normalized and plotted.

  • Cell line analysis for total AKT1 from 20 µg/mL preparations of cell extracts. Data from triplicate measurements (mean +/- SD) are plotted and compared to 1X Cell Extraction Buffer PTR (zero).

  • Induction of AKT (pT308) phosphorylation in MCF-7 cells in response to insulin treatment. MCF-7 cells were cultured in 96-well tissue culture plates, serum-starved and treated (5 min) with a dose-range of insulin before cell lysis. Data from quadruplicate measurements of AKT (pT308) are plotted and compared against total AKT1 protein levels. Comparative AKT (pT308) and AKT1 (Total) data also shown by Western Blot.


References for AKT 1/2/3 pT308 + AKT1 ELISA Kit (ab176658)

ab176658 has not yet been referenced specifically in any publications.

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