AKT Activity Assay Kit (ab65786)
- Product nameAKT Activity Assay KitSee all AKT kits ...
- Tests40 x 1 assay
- Sample typeTissue Extracts, Cell Lysate
- Assay typeEnzyme activity
- Product overview
Abcam's AKT Activity Assay Kit utilizes an Akt-specific antibody to immunoprecipitate Akt from cell lysate. Activity of the Akt is then determined in a kinase reaction using recombinant GSK-3α as substrate. Phosphorylation of the GSK-3α can be analyzed by Western blot analysis using the phospho-GSK-3α specific antibody included in the kit. The kit specifically detects Akt1, Akt2, and Akt3 activities, other kinase activities would not be detected.
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The AKT Specific Antibody reacts with human, mouse, and rat.
Akt is a protein kinase that can be activated by insulin and various growth factors and functions in a pathway involving PI3 kinase. Recent evidence suggests that Akt functions to promote cell survival by actively inhibiting apoptosis.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 40 tests Akt Specific Antibody Red 1 x 80µl GSK-3a Protein/ATP Mixture Blue 1 x 80µl Kinase Assay Buffer WM 1 x 25ml Kinase Extraction Buffer NM 1 x 80ml Phospho-GSK-3a Specific Antibody Green 1 x 50µl Protein A Sepharose Clear 1 x 2ml
- Research Areas
- AKT1C AKTPKB
- PRKBARACRAC alphaRAC alpha serine/threonine-protein kinaseRAC PK alphav akt murine thymoma viral oncogene homolog 1
Our Abpromise guarantee covers the use of ab65786 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
AKT Activity Assay Kit images
Western blot analysis of phospho-GSK-3 alpha in AKT negative (Lane 1) and AKT positive (Lane 2,3) Jurkat cell lysates using AKT Activity Assay Kit (ab65786).
References for AKT Activity Assay Kit (ab65786)
This product has been referenced in:
- Damera G et al. An RGS4-mediated phenotypic switch of bronchial smooth muscle cells promotes fixed airway obstruction in asthma. PLoS One 7:e28504 (2012). Functional Studies . Read more (PubMed: 22253691) »
- Miyazaki M et al. Early activation of mTORC1 signalling in response to mechanical overload is independent of phosphoinositide 3-kinase/Akt signalling. J Physiol 589:1831-46 (2011). Read more (PubMed: 21300751) »