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Abcam's AKT Activity Assay Kit utilizes an Akt-specific antibody to immunoprecipitate Akt from cell lysate. Activity of the Akt is then determined in a kinase reaction using recombinant GSK-3a as substrate. Phosphorylation of the GSK-3a can be analyzed by Western blot analysis using the phospho-GSK-3a specific antibody included in the kit. The kit specifically detects Akt1, Akt2, and Akt3 activities, other kinase activities would not be detected.
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The AKT Specific Antibody reacts with human, mouse, and rat.
Akt is a protein kinase that can be activated by insulin and various growth factors and functions in a pathway involving PI3 kinase. Recent evidence suggests that Akt functions to promote cell survival by actively inhibiting apoptosis.
|Akt Specific Antibody||Red||1 x 80µl|
|GSK-3a Protein/ATP Mixture||Blue||1 x 80µl|
|Kinase Assay Buffer||WM||1 x 25ml|
|Kinase Extraction Buffer||NM||1 x 80ml|
|Phospho-GSK-3a Specific Antibody||Green||1 x 50µl|
|Protein A Sepharose||Clear||1 x 2ml|
Our Abpromise guarantee covers the use of ab65786 in the following tested applications.
|Functional Studies||Use at an assay dependent dilution.|