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Product name
Anti-AKT1/2/3 antibody
See all AKT1/2/3 products (4) ...
Description
Rabbit polyclonal to AKT1/2/3
Tested applications
WB, IP, ELISA, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Chicken, Human
Predicted to work with
Sheep, Goat, Cow, Dog, Pig
Immunogen
Synthetic peptide: CRPHFPQFSYSASGTA conjugated to KLH using maleimide, corresponding to C terminal amino acids 466-480 of Human, Mouse, Rat and Chicken AKT1/2/3.
CRPHFPQFSYSASGTA
Positive control
Human colon tumour tissue, Human liver tissue, Cultured neonatal rat cardiomyocytes expressing an AKT construct
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
Concentration
Concentration information loading...
Purification notes
Delipidation and Defibrination
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> AKT
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKB / AKT
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AKT1/2/3 antibody (ab106693)
(enlarge)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AKT1/2/3 antibody (ab106693)
(enlarge)
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Immunocytochemistry/ Immunofluorescence - Anti-AKT1/2/3 antibody (ab106693)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab106693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/2000. Predicted molecular weight: 56 kDa.Can be blocked with AKT1 peptide (466/480) (ab9041).
IP: Use at an assay dependent dilution.
ELISA: 1/2000 - 1/10000.
IHC-P: 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF: 1/100 - 1/1000.
Target
Function
IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
Tissue specificity
In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Domain
Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
Post-translational
modifications
Phosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
Cellular localization
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
Target information above from: UniProt accessionQ9Y243
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DKFZp434N0250 antibody
- MGC99656 antibody
- AKT antibody
see all
Database links
- Entrez Gene: 208 Human
- Entrez Gene: 207 Human
- Entrez Gene: 10000 Human
- Entrez Gene: 11651 Mouse
- Entrez Gene: 11652 Mouse
- Entrez Gene: 23797 Mouse
- Entrez Gene: 24185 Rat
- Entrez Gene: 25233 Rat
see all
Anti-AKT1/2/3 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AKT1/2/3 antibody (ab106693)
ab106693, at 1/100 dilution, staing AKT1/2/3 in Human liver followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AKT1/2/3 antibody (ab106693)
ab106693 at 1/100 dilution, staining AKT1/2/3 in Human colon, A) normal tissue B) tumour tissue.
Immunocytochemistry/ Immunofluorescence - Anti-AKT1/2/3 antibody (ab106693)
ab106693, at 1/80 dilution, staining AKT1/2/3 in cultured neonatal Rat cardiomyocytes that express a nuclear-targeted AKT construct. Anti-AKT1/2/3 staining appears green. Actin filaments are labeled red using a Texas-red™ conjugated phalloidin.
References for Anti-AKT1/2/3 antibody (ab106693)
ab106693 has not yet been referenced specifically in any publications.
Publishing research using ab106693? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"