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- Secondary antibodies
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Product name
Anti-AKT1/2/3 antibody
See all AKT1/2/3 products (4) ...
Description
Rabbit polyclonal to AKT1/2/3
Specificity
ab82538 recognizes AKT1, AKT2, and AKT3.
Tested applications
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide, corresponding to amino acids 471-475 of Human AKT1/2/3
Positive control
Human recombinant AKT1, AKT2, and AKT3; HeLa, HEK-293T, Jurkat, THP1, and 3T6 cell lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> AKT
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKB / AKT
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab82538 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000. Predicted molecular weight: 56 kDa.
Target
Function
IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
Tissue specificity
In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Domain
Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
Post-translational
modifications
Phosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
Cellular localization
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
Target information above from: UniProt accessionQ9Y243
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DKFZp434N0250 antibody
- MGC99656 antibody
- AKT antibody
see all
Database links
- Entrez Gene: 208 Human
- Entrez Gene: 207 Human
- Entrez Gene: 10000 Human
- Entrez Gene: 11651 Mouse
- Entrez Gene: 11652 Mouse
- Entrez Gene: 23797 Mouse
- Omim: 164730 Human
- Omim: 164731 Human
see all
Anti-AKT1/2/3 antibody images:
Western blot - AKT1/2/3 antibody (ab82538)
All lanes : Anti-AKT1/2/3 antibody (ab82538) at 1/1000 dilution
Lane 1 : human recombinant AKT1
Lane 2 : human recombinant AKT2
Lane 3 : human recombinant AKT3
Lysates/proteins at 0.02 µg per lane.
Predicted band size : 56 kDa
Observed band size : 85 kDa (why is the actual band size different from the predicted?)
Western blot - AKT1/2/3 antibody (ab82538)
All lanes : Anti-AKT1/2/3 antibody (ab82538) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HEK-293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : THP1 cell lysate
Lane 5 : 3T6 cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size : 56 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
References for Anti-AKT1/2/3 antibody (ab82538)
ab82538 has not yet been referenced specifically in any publications.
Publishing research using ab82538? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"