Overview
- Product nameAnti-AKT1/2 antibodySee all AKT1/2 primary antibodies ...
- DescriptionRabbit polyclonal to AKT1/2
- Tested applicationsWB, IP, IHC-P more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Synthetic peptide (Human) conjugated to KLH
- Positive controlWB: mouse or Rat brain lysate; HeLa or Hs-67 cell lysate. IHC-P: heart, prostate or thymus tissue.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Store at -20ºC.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS -
Concentration information loading... - PurityProtein A purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab109870 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/1000. Predicted molecular weight: 56 kDa. |
| IP | IP: Use at an assay dependent dilution. |
| IHC-P | IHC-P: Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
- RelevanceThe serine/threonine kinase AKT (protein kinase B or PKB) has a central role in the regulation of several signaling pathways controlling cell proliferation, apoptosis, angiogenesis, and diabetes. In humans, there are three genes in the "AKT family": AKT1, AKT2, and AKT3. AKT1 is catalytically inactive in serum starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet derived growth factor. The activation is rapid and specific. In the developing nervous system AKT is a critical mediator of growth factor induced neuronal survival. Survival factors can suppress apoptosis in a transcription independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. AKT2 is a putative oncogene and is a general protein kinase capable of phophorylating several known proteins. AKT2 is amplified and overexpressed in some human carcinomas. AKT2 acts primarily as a regulator of glucose metabolism.
- Cellular localizationATK1: Cytoplasm. Nucleus. Cell membrane. Note: Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A.
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Database links
- Entrez Gene: 207 Human
- Entrez Gene: 208 Human
- Entrez Gene: 11651 Mouse
- Entrez Gene: 11652 Mouse
- Entrez Gene: 24185 Rat
- Entrez Gene: 25233 Rat
- Omim: 164730 Human
- Omim: 164731 Human
- SwissProt: P31749 Human
- SwissProt: P31751 Human
- SwissProt: P31750 Mouse
- SwissProt: Q60823 Mouse
- SwissProt: P47196 Rat
- SwissProt: P47197 Rat
- Unigene: 525622 Human
- Unigene: 631535 Human
see all
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Alternative names
- AKT 1 antibodyAKT 2 antibodyAKT antibody
- AKT1 antibodyAKT1 kinase antibodyAKT2 antibodyAKT2 kinase antibodyC AKT antibodyMGC99656 antibodyMurine thymoma viral (v akt) homolog 2 antibodyOncogene AKT1 antibodyPKB alpha antibodyPKB antibodyPKB beta antibodyPKBB antibodyPKBBETA antibodyPRKBA antibodyPRKBB antibodyProtein kinase Akt 2 antibodyProtein Kinase B Alpha antibodyProtein kinase B antibodyProtein kinase B beta antibodyRAC antibodyRAC alpha antibodyRAC alpha serine/threonine protein kinase antibodyRAC BETA antibodyRAC beta serine threonine protein kinase antibodyRAC PK alpha antibodyRAC PK beta antibodyRac protein kinase alpha antibodyRac protein kinase beta antibodyRACbeta antibodyv akt murine thymoma viral oncogene homolog 1 antibodyv akt murine thymoma viral oncogene homolog 2 antibody
see all
Anti-AKT1/2 antibody images
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ab109870 at 10 µg/ml staining AKT1/2 in formalin-fixed, paraffin-embedded Human heart tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
After incubation with the primary antibody, slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. -
ab109870 at 10 µg/ml staining AKT1/2 in formalin-fixed, paraffin-embedded Human placenta (red blood cells) after heat induced antigen retrieval in pH 6.0 citrate buffer.
After incubation with the primary antibody, slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. -
ab109870 at 10 µg/ml staining AKT1/2 in formalin-fixed, paraffin-embedded Human prostate tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
After incubation with the primary antibody, slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. -
ab109870 at 10 µg/ml staining AKT1/2 in formalin-fixed, paraffin-embedded Human thymus tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
After incubation with the primary antibody, slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
References for Anti-AKT1/2 antibody (ab109870)
ab109870 has not yet been referenced specifically in any publications.



