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Product name
Anti-AKT1/2 antibody
See all AKT1/2 products (2) ...
Description
Rabbit polyclonal to AKT1/2
Tested applications
WB, IP, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide (Human) conjugated to KLH
Positive control
WB: mouse or Rat brain lysate; HeLa or Hs-67 cell lysate. IHC-P: heart, prostate or thymus tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20ºC.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKB / AKT
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AKT1/2 antibody (ab109870)
(enlarge)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AKT1/2 antibody (ab109870)
(enlarge)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AKT1/2 antibody (ab109870)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab109870 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000. Predicted molecular weight: 56 kDa.
IP: Use at an assay dependent dilution.
IHC-P: Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Target
Relevance
The serine/threonine kinase AKT (protein kinase B or PKB) has a central role in the regulation of several signaling pathways controlling cell proliferation, apoptosis, angiogenesis, and diabetes. In humans, there are three genes in the "AKT family": AKT1, AKT2, and AKT3. AKT1 is catalytically inactive in serum starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet derived growth factor. The activation is rapid and specific. In the developing nervous system AKT is a critical mediator of growth factor induced neuronal survival. Survival factors can suppress apoptosis in a transcription independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. AKT2 is a putative oncogene and is a general protein kinase capable of phophorylating several known proteins. AKT2 is amplified and overexpressed in some human carcinomas. AKT2 acts primarily as a regulator of glucose metabolism.
Cellular localization
ATK1: Cytoplasm. Nucleus. Cell membrane. Note: Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A.
Alternative names
- PKB antibody
- RAC PK alpha antibody
- AKT 1 antibody
see all
Database links
- Entrez Gene: 207 Human
- Entrez Gene: 208 Human
- Entrez Gene: 11651 Mouse
- Entrez Gene: 11652 Mouse
- Entrez Gene: 24185 Rat
- Entrez Gene: 25233 Rat
- Omim: 164730 Human
- Omim: 164731 Human
see all
Anti-AKT1/2 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AKT1/2 antibody (ab109870)
ab109870 at 10 µg/ml staining AKT1/2 in formalin-fixed, paraffin-embedded Human heart tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
After incubation with the primary antibody, slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AKT1/2 antibody (ab109870)
ab109870 at 10 µg/ml staining AKT1/2 in formalin-fixed, paraffin-embedded Human placenta (red blood cells) after heat induced antigen retrieval in pH 6.0 citrate buffer.
After incubation with the primary antibody, slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AKT1/2 antibody (ab109870)
ab109870 at 10 µg/ml staining AKT1/2 in formalin-fixed, paraffin-embedded Human prostate tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
After incubation with the primary antibody, slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AKT1/2 antibody (ab109870)
ab109870 at 10 µg/ml staining AKT1/2 in formalin-fixed, paraffin-embedded Human thymus tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
After incubation with the primary antibody, slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
References for Anti-AKT1/2 antibody (ab109870)
ab109870 has not yet been referenced specifically in any publications.
Publishing research using ab109870? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"