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Synthetic peptide (Human)
This product was manufactured under U.S. Patent No. 5,675,063.
Our Abpromise guarantee covers the use of ab108202 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 56 kDa.|
Lanes 1, 3 and 5: Wild-type HAP1 cell lysate (20 µg)
Lanes 2, 4 and 6: AKT1 knockout HAP1 cell lysate (20 µg)
Lanes 1 and 2: Green signal from target - ab108202 observed at 55 kDa
Lanes 3 and 4: Red signal from loading control - ab8227 observed at 42 kDa
Lanes 5 and 6: Merged (red and green) signal
ab108202 was shown to specifically react with AKT1 when AKT1 knockout samples were used. Wild-type and AKT1 knockout samples were subjected to SDS-PAGE. ab108202 and ab8227 (loading control to beta actin) were both diluted at 1/1000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
ab108202 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"