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Overview

  • Product nameAnti-AKT1 antibodySee all AKT1 primary antibodies ...
  • Description
    Rabbit polyclonal to AKT1
  • Specificityab64936 detects endogenous levels of total AKT1 protein.
  • Tested applicationsELISA, WB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (Human) from the N terminal region.

  • Positive controlextracts from 293 and HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab64936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA ELISA: 1/5000.
WB WB: 1/500 - 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).

Target

  • FunctionPlays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation (By similarity). General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. The activated form can suppress FoxO gene transcription and promote cell cycle progression. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.
  • Tissue specificityExpressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.
  • Involvement in diseaseDefects in AKT1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
    Defects in AKT1 are associated with colorectal cancer (CRC) [MIM:114500].
    Defects in AKT1 are associated with susceptibility to ovarian cancer [MIM:604370]; also called susceptibility to familial breast-ovarian cancer type 1 (BROVCA1).
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • DomainBinding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane. The PH domain mediates interaction with TNK2 and Tyr-176 is also essential for this interaction.
    The AGC-kinase C-terminal mediates interaction with THEM4.
  • Post-translational
    modifications    Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Activated TNK2 phosphorylates it on Tyr-176 resulting in its binding to the anionic plasma membrane phospholipid PA. This phosphorylated form localizes to the cell membrane, where it is targeted by PDPK1 and PDPK2 for further phosphorylations on Thr-308 and Ser-473 leading to its activation. Ser-473 phosphorylation by mTORC2 favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with AGAP2 isoform 2 (PIKE-A). Ser-473 phosphorylation is enhanced in focal cortical dysplasias with Taylor-type balloon cells.
    Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT1 ubiquitination is critical for phosphorylation and activation. When ubiquitinated, it translocates to the plasma membrane, where it becomes phosphorylated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
  • Cellular localizationCytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus.

    • Target information above from: UniProt accession P31749 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      AKT 1 antibodyAKT antibodyAKT1 antibody
      AKT1_HUMAN antibodyC AKT antibodycAKT antibodyMGC9965 antibodyMGC99656 antibodyOncogene AKT1 antibodyPKB antibodyPKB-ALPHA antibodyPRKBA antibodyProtein Kinase B Alpha antibodyProtein kinase B antibodyProto-oncogene c-Akt antibodyRAC Alpha antibodyRAC alpha serine/threonine protein kinase antibodyRAC antibodyRAC PK Alpha antibodyRac protein kinase alpha antibodyRAC Serine/Threonine Protein Kinase antibodyRAC-alpha serine/threonine-protein kinase antibodyRAC-PK-alpha antibodyv akt murine thymoma viral oncogene homolog 1 antibodyvAKT Murine Thymoma Viral Oncogene Homolog 1 antibody
    see all

Anti-AKT1 antibody images

  • Western blot - AKT1 antibody (ab64936)
    Western blot - AKT1 antibody (ab64936)
    All lanes : Anti-AKT1 antibody (ab64936) at 1/500 dilution

    Lane 1 : extracts from 293 cells (5-30µg total protein)
    Lane 2 : extracts from HepG2 cells (5-30µg total protein)
    Lane 3 : extracts from HepG2 cells (5-30µg total protein) and 5-10µg of the immunising peptide.


    Predicted band size : 56 kDa
    Observed band size : 56 kDa
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

References for Anti-AKT1 antibody (ab64936)

ab64936 has not yet been referenced specifically in any publications.

Publishing research using ab64936 ? Please let us know so that we can cite the reference in this datasheet

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Thank you, that is a great help!

You are welcome.
Do not hesitate to contact us againif you need any more information.
Good luck with your research.

Hi,

I was hoping for some advice regarding your antibodies against both AKT 1 or AKT 2 (separately not pan-AKT). I am interested in blotting for truncated mutants of both and therefore need Abs that recognise sequence as near as possible to the N...

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Thank you for contacting us.

All the products you shortlisted seem to be suitable for your work, however, looking at the availability of those products, I would recommend the following one that are currently in stock:
Anti-AKT1 (N-term)antibo...

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